Distinct properties underlie flavin-based electron bifurcation in a novel electron transfer flavoprotein FixAB from Rhodopseudomonas palustris

A newly-recognized third fundamental mechanism of energy conservation in biology, electron bifurcation, uses free energy from exergonic redox reactions to drive endergonic redox reactions. Flavin-based electron bifurcation furnishes low potential electrons to demanding chemical reactions such as red...

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Bibliographic Details
Published in:The Journal of biological chemistry 2018-03, Vol.293 (13)
Main Authors: Duan, H. Diessel, Lubner, Carolyn E., Tokmina-Lukaszewska, Monika, Gauss, George H., Bothner, Brian, King, Paul W., Peters, John W., Miller, Anne-Frances
Format: Article
Language:English
Subjects:
BASIC BIOLOGICAL SCIENCES
circular dichroism
electron bifurcation
electron transfer
electron transfer flavorprotein
ETF
FixAB
flavin
flavorprotein
nitrogenase
reduction midpoint potential
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Summary:A newly-recognized third fundamental mechanism of energy conservation in biology, electron bifurcation, uses free energy from exergonic redox reactions to drive endergonic redox reactions. Flavin-based electron bifurcation furnishes low potential electrons to demanding chemical reactions such as reduction of dinitrogen to ammonia. We employed the heterodimeric flavoenzyme FixAB from the diazotrophic bacterium Rhodopseudomonas palustris to elucidate unique properties that underpin flavin-based electron bifurcation.
ISSN:0021-9258
1083-351X