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Immobilization of Methylosinus trichosporium OB3b for methanol production

Abstract Due to the natural gas boom in North America, there is renewed interest in the production of other chemical products from methane. We investigated the feasibility of immobilizing the obligate methanotrophic bacterium Methylosinus trichosporium OB3b in alginate beads, and selectively inactiv...

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Published in:Journal of industrial microbiology & biotechnology 2018-03, Vol.45 (3)
Main Authors: Taylor, Anne, Molzahn, Paige, Bushnell, Tanner, Cheney, Clint, LaJeunesse, Monique, Azizian, Mohamad, Semprini, Lewis
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container_title Journal of industrial microbiology & biotechnology
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creator Taylor, Anne
Molzahn, Paige
Bushnell, Tanner
Cheney, Clint
LaJeunesse, Monique
Azizian, Mohamad
Semprini, Lewis
description Abstract Due to the natural gas boom in North America, there is renewed interest in the production of other chemical products from methane. We investigated the feasibility of immobilizing the obligate methanotrophic bacterium Methylosinus trichosporium OB3b in alginate beads, and selectively inactivating methanol dehydrogenase (MDH) with cyclopropane to produce methanol. In batch cultures and in semi-continuous flow columns, the exposure of alginate-immobilized cells to cyclopropane or cyclopropanol resulted in the loss of the majority of MDH activity (> 80%), allowing methanol to accumulate to significant concentrations while retaining all of M. trichosporium OB3b’s methane monooxygenase capacity. Thereafter, the efficiency of methanol production fell due to recovery of most of the MDH activity; however, subsequent inhibition periods resulted in renewed methanol production efficiency, and immobilized cells retained methane-oxidizing activity for at least 14 days.
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title Immobilization of Methylosinus trichosporium OB3b for methanol production
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