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Immobilization of Methylosinus trichosporium OB3b for methanol production
Abstract Due to the natural gas boom in North America, there is renewed interest in the production of other chemical products from methane. We investigated the feasibility of immobilizing the obligate methanotrophic bacterium Methylosinus trichosporium OB3b in alginate beads, and selectively inactiv...
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Published in: | Journal of industrial microbiology & biotechnology 2018-03, Vol.45 (3) |
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container_title | Journal of industrial microbiology & biotechnology |
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creator | Taylor, Anne Molzahn, Paige Bushnell, Tanner Cheney, Clint LaJeunesse, Monique Azizian, Mohamad Semprini, Lewis |
description | Abstract
Due to the natural gas boom in North America, there is renewed interest in the production of other chemical products from methane. We investigated the feasibility of immobilizing the obligate methanotrophic bacterium Methylosinus trichosporium OB3b in alginate beads, and selectively inactivating methanol dehydrogenase (MDH) with cyclopropane to produce methanol. In batch cultures and in semi-continuous flow columns, the exposure of alginate-immobilized cells to cyclopropane or cyclopropanol resulted in the loss of the majority of MDH activity (> 80%), allowing methanol to accumulate to significant concentrations while retaining all of M. trichosporium OB3b’s methane monooxygenase capacity. Thereafter, the efficiency of methanol production fell due to recovery of most of the MDH activity; however, subsequent inhibition periods resulted in renewed methanol production efficiency, and immobilized cells retained methane-oxidizing activity for at least 14 days. |
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Due to the natural gas boom in North America, there is renewed interest in the production of other chemical products from methane. We investigated the feasibility of immobilizing the obligate methanotrophic bacterium Methylosinus trichosporium OB3b in alginate beads, and selectively inactivating methanol dehydrogenase (MDH) with cyclopropane to produce methanol. In batch cultures and in semi-continuous flow columns, the exposure of alginate-immobilized cells to cyclopropane or cyclopropanol resulted in the loss of the majority of MDH activity (> 80%), allowing methanol to accumulate to significant concentrations while retaining all of M. trichosporium OB3b’s methane monooxygenase capacity. Thereafter, the efficiency of methanol production fell due to recovery of most of the MDH activity; however, subsequent inhibition periods resulted in renewed methanol production efficiency, and immobilized cells retained methane-oxidizing activity for at least 14 days.</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><language>eng</language><publisher>Germany: Oxford University Press</publisher><ispartof>Journal of industrial microbiology & biotechnology, 2018-03, Vol.45 (3)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,778,782,883</link.rule.ids><backlink>$$Uhttps://www.osti.gov/biblio/1773507$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Taylor, Anne</creatorcontrib><creatorcontrib>Molzahn, Paige</creatorcontrib><creatorcontrib>Bushnell, Tanner</creatorcontrib><creatorcontrib>Cheney, Clint</creatorcontrib><creatorcontrib>LaJeunesse, Monique</creatorcontrib><creatorcontrib>Azizian, Mohamad</creatorcontrib><creatorcontrib>Semprini, Lewis</creatorcontrib><title>Immobilization of Methylosinus trichosporium OB3b for methanol production</title><title>Journal of industrial microbiology & biotechnology</title><description>Abstract
Due to the natural gas boom in North America, there is renewed interest in the production of other chemical products from methane. We investigated the feasibility of immobilizing the obligate methanotrophic bacterium Methylosinus trichosporium OB3b in alginate beads, and selectively inactivating methanol dehydrogenase (MDH) with cyclopropane to produce methanol. In batch cultures and in semi-continuous flow columns, the exposure of alginate-immobilized cells to cyclopropane or cyclopropanol resulted in the loss of the majority of MDH activity (> 80%), allowing methanol to accumulate to significant concentrations while retaining all of M. trichosporium OB3b’s methane monooxygenase capacity. Thereafter, the efficiency of methanol production fell due to recovery of most of the MDH activity; however, subsequent inhibition periods resulted in renewed methanol production efficiency, and immobilized cells retained methane-oxidizing activity for at least 14 days.</description><issn>1367-5435</issn><issn>1476-5535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqNi7sKwjAUQIMoWB__ENwLrWmaXVHsIC7upY0pvZLkltx00K-3gh_gdM5wzowleaHKVEoh55OLUqWyEHLJVkTPLMukUvuEVZVz2IKFdxMBPceOX03sXxYJ_Eg8BtA90oABRsdvB9HyDgN3U9N4tHwI-Bj1d92wRddYMtsf12x3Pt2PlxQpQk0aotG9Ru-NjnWulJCZEn9FH5T_Pp0</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Taylor, Anne</creator><creator>Molzahn, Paige</creator><creator>Bushnell, Tanner</creator><creator>Cheney, Clint</creator><creator>LaJeunesse, Monique</creator><creator>Azizian, Mohamad</creator><creator>Semprini, Lewis</creator><general>Oxford University Press</general><scope>OTOTI</scope></search><sort><creationdate>20180301</creationdate><title>Immobilization of Methylosinus trichosporium OB3b for methanol production</title><author>Taylor, Anne ; Molzahn, Paige ; Bushnell, Tanner ; Cheney, Clint ; LaJeunesse, Monique ; Azizian, Mohamad ; Semprini, Lewis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-osti_scitechconnect_17735073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taylor, Anne</creatorcontrib><creatorcontrib>Molzahn, Paige</creatorcontrib><creatorcontrib>Bushnell, Tanner</creatorcontrib><creatorcontrib>Cheney, Clint</creatorcontrib><creatorcontrib>LaJeunesse, Monique</creatorcontrib><creatorcontrib>Azizian, Mohamad</creatorcontrib><creatorcontrib>Semprini, Lewis</creatorcontrib><collection>OSTI.GOV</collection><jtitle>Journal of industrial microbiology & biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taylor, Anne</au><au>Molzahn, Paige</au><au>Bushnell, Tanner</au><au>Cheney, Clint</au><au>LaJeunesse, Monique</au><au>Azizian, Mohamad</au><au>Semprini, Lewis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immobilization of Methylosinus trichosporium OB3b for methanol production</atitle><jtitle>Journal of industrial microbiology & biotechnology</jtitle><date>2018-03-01</date><risdate>2018</risdate><volume>45</volume><issue>3</issue><issn>1367-5435</issn><eissn>1476-5535</eissn><abstract>Abstract
Due to the natural gas boom in North America, there is renewed interest in the production of other chemical products from methane. We investigated the feasibility of immobilizing the obligate methanotrophic bacterium Methylosinus trichosporium OB3b in alginate beads, and selectively inactivating methanol dehydrogenase (MDH) with cyclopropane to produce methanol. In batch cultures and in semi-continuous flow columns, the exposure of alginate-immobilized cells to cyclopropane or cyclopropanol resulted in the loss of the majority of MDH activity (> 80%), allowing methanol to accumulate to significant concentrations while retaining all of M. trichosporium OB3b’s methane monooxygenase capacity. Thereafter, the efficiency of methanol production fell due to recovery of most of the MDH activity; however, subsequent inhibition periods resulted in renewed methanol production efficiency, and immobilized cells retained methane-oxidizing activity for at least 14 days.</abstract><cop>Germany</cop><pub>Oxford University Press</pub></addata></record> |
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title | Immobilization of Methylosinus trichosporium OB3b for methanol production |
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