Heterogeneous isotope effects decouple conifer leaf and branch sugar δ18O and δ13C

• Published in: Oecologia 2022-02, Vol.198 (2), p.357-370
• Main Authors: Fiorella, Richard P., Kannenberg, Steven A., Anderegg, William R. L., Monson, Russell K., Ehleringer, James R.
• Format: Article
• Language: English
• Subjects: BASIC BIOLOGICAL SCIENCES; Biomedical and Life Sciences; Carbohydrates; Cellulose; Earth Sciences; Ecology; Environment models; Exports; Heterogeneity; Hydrology/Water Resources; Isotope ratios; Isotopes; Leaves; Life Sciences; Organic matter; Oxygen exchange; Paleoclimate; Patchiness; Phloem; Photosynthesis; Physiological Ecology–Original Research; Pine needles; Pine trees; Pinus ponderosa; Plant Sciences; Ponderosa pine; Ratios; Saccharides; Spatial heterogeneity; stable isotopes; Sugar; sugars; Tree rings
• ISSN: 0029-8549; 1432-1939
• DOI: 10.1007/s00442-022-05121-y

Isotope ratios of tree-ring cellulose are a prominent tool to reconstruct paleoclimate and plant responses to environmental variation. Current models for cellulose isotope ratios assume a transfer of the environmental signals recorded in bulk leaf water to carbohydrates and ultimately into stem cellulose. However, the isotopic signal of carbohydrates exported from leaf to branch may deviate from mean leaf values if spatial heterogeneity in isotope ratios exists in the leaf. We tested whether the isotopic heterogeneity previously observed along the length of a ponderosa pine ( Pinus ponderosa ) leaf water was preserved in photosynthetic products. We observed an increase in both sugar and bulk tissue δ 18 O values along the needle, but the increase in carbohydrate δ 18 O values was dampened relative to the trend observed in leaf water. In contrast, δ 13 C values of both sugar and bulk organic matter were invariant along the needle. Phloem-exported sugar measured in the branch below the needles did not match whole-needle values of δ 18 O or δ 13 C. Instead, there was a near-constant offset observed between the branch and needle sugar δ 13 C values, while branch δ 18 O values were most similar to δ 18 O values observed for sugar at the base of the needle. The observed offset between the branch and needle sugar δ 18 O values likely arises from partial isotope oxygen exchange between sugars and water during phloem loading and transport. An improved understanding of the conditions producing differential δ 13 C and δ 18 O isotope effects between branch phloem and needle sugars could improve tree-ring-based climate reconstructions.