Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes

A size selective nanorattle was formed by encapsulating soybean peroxidase (SBP) within a ZnS mesoporous hollow sphere. Once encapsulated within the mesoporous hollow sphere, the SBP remained active against molecules smaller than the 3 nm diameter of the mesopores in the shell wall, while molecules...

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Bibliographic Details
Published in:Nano letters 2009-05, Vol.9 (5), p.1994-1998
Main Authors: Van Gough, Dara, Wolosiuk, Alejandro, Braun, Paul V
Format: Article
Language:English
Subjects:
BASIC BIOLOGICAL SCIENCES
Catalysis
Catalytic methods
Colloids
Cross-disciplinary physics: materials science
rheology
Encapsulation
Enzymes, Immobilized - chemistry
Exact sciences and technology
Fluorescence
Glycine max - enzymology
Hydrogen Peroxide - chemistry
Materials science
Methods of nanofabrication
Nanoparticles - chemistry
Oxazines - chemistry
Particle Size
Peptides and proteins
Peroxidase - chemistry
Physics
Porosity
Reagents
Sodium Azide - chemistry
Sulfides - chemistry
Zinc Compounds - chemistry
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Summary:A size selective nanorattle was formed by encapsulating soybean peroxidase (SBP) within a ZnS mesoporous hollow sphere. Once encapsulated within the mesoporous hollow sphere, the SBP remained active against molecules smaller than the 3 nm diameter of the mesopores in the shell wall, while molecules larger than the mesopores, which could not pass into the hollow sphere, did not interact with the SBP. Specifically, encapsulated SBP catalyzed the oxidation of Amplex Ultra-Red, a small fluorogen, in the presence of hydrogen peroxide, encapsulated SBP was deactivated by sodium azide, and no reaction was observed between encapsulated SBP and a greater than 3 nm diameter protease.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl900264n