Loading…
Microbial production of advanced biofuels
Concerns over climate change have necessitated a rethinking of our transportation infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced by engineered microorganisms that use a renewable carbon source. Two biofuels, ethanol and biodiesel, have made inroads in...
Saved in:
| Published in: | Nature reviews. Microbiology 2021-11, Vol.19 (11), p.701-715 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c446t-c564e54a3f35eab20e8c74f086e74fff130cfd03426fca4f3c465d0b1cf75db93 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c446t-c564e54a3f35eab20e8c74f086e74fff130cfd03426fca4f3c465d0b1cf75db93 |
| container_end_page | 715 |
| container_issue | 11 |
| container_start_page | 701 |
| container_title | Nature reviews. Microbiology |
| container_volume | 19 |
| creator | Keasling, Jay Garcia Martin, Hector Lee, Taek Soon Mukhopadhyay, Aindrila Singer, Steven W. Sundstrom, Eric |
| description | Concerns over climate change have necessitated a rethinking of our transportation infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced by engineered microorganisms that use a renewable carbon source. Two biofuels, ethanol and biodiesel, have made inroads in displacing petroleum-based fuels, but their uptake has been limited by the amounts that can be used in conventional engines and by their cost. Advanced biofuels that mimic petroleum-based fuels are not limited by the amounts that can be used in existing transportation infrastructure but have had limited uptake due to costs. In this Review, we discuss engineering metabolic pathways to produce advanced biofuels, challenges with substrate and product toxicity with regard to host microorganisms and methods to engineer tolerance, and the use of functional genomics and machine learning approaches to produce advanced biofuels and prospects for reducing their costs.
Biofuels produced by conversion of biomass by engineered microorganisms have the potential to replace fossil fuels and reduce carbon emissions. In this Review, Keasling and colleagues discuss engineering of metabolic pathways to produce advanced biofuels and approaches to reduce metabolite toxicity and cost and increase titre, rate and yield. |
| doi_str_mv | 10.1038/s41579-021-00577-w |
| format | article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_1902663</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2580827914</sourcerecordid><originalsourceid>FETCH-LOGICAL-c446t-c564e54a3f35eab20e8c74f086e74fff130cfd03426fca4f3c465d0b1cf75db93</originalsourceid><addsrcrecordid>eNp9kD1PwzAQhi0EoqXwBxhQBBND4PydjKjiSypigdlyHBtStXGxk1b8ewwpZWM6S_fce-cHoVMMVxhocR0Z5rLMgeAcgEuZb_bQGEsGOeaU7e_eRIzQUYxzAMK5JIdoRBmWpOR4jC6fGhN81ehFtgq-7k3X-DbzLtP1WrfG1lnVeNfbRTxGB04voj3Z1gl6vbt9mT7ks-f7x-nNLDeMiS43XDDLmaaOcqsrArYwkjkohE3FOUzBuBooI8IZzRw1TPAaKmyc5HVV0gk6H3J97BoVTdNZ825821rTKVwCEYIm6GKA0tEfvY2dmvs-tOkuRXgBBZElZokiA5W-GGOwTq1Cs9ThU2FQ3wrVoFAlhepHodqkobNtdF8tbb0b-XWWADoAMbXaNxv-dv8T-wVAdXtT</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2580827914</pqid></control><display><type>article</type><title>Microbial production of advanced biofuels</title><source>North East Research Libraries Nature Academic Titles</source><creator>Keasling, Jay ; Garcia Martin, Hector ; Lee, Taek Soon ; Mukhopadhyay, Aindrila ; Singer, Steven W. ; Sundstrom, Eric</creator><creatorcontrib>Keasling, Jay ; Garcia Martin, Hector ; Lee, Taek Soon ; Mukhopadhyay, Aindrila ; Singer, Steven W. ; Sundstrom, Eric ; Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)</creatorcontrib><description>Concerns over climate change have necessitated a rethinking of our transportation infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced by engineered microorganisms that use a renewable carbon source. Two biofuels, ethanol and biodiesel, have made inroads in displacing petroleum-based fuels, but their uptake has been limited by the amounts that can be used in conventional engines and by their cost. Advanced biofuels that mimic petroleum-based fuels are not limited by the amounts that can be used in existing transportation infrastructure but have had limited uptake due to costs. In this Review, we discuss engineering metabolic pathways to produce advanced biofuels, challenges with substrate and product toxicity with regard to host microorganisms and methods to engineer tolerance, and the use of functional genomics and machine learning approaches to produce advanced biofuels and prospects for reducing their costs.
Biofuels produced by conversion of biomass by engineered microorganisms have the potential to replace fossil fuels and reduce carbon emissions. In this Review, Keasling and colleagues discuss engineering of metabolic pathways to produce advanced biofuels and approaches to reduce metabolite toxicity and cost and increase titre, rate and yield.</description><identifier>ISSN: 1740-1526</identifier><identifier>EISSN: 1740-1534</identifier><identifier>DOI: 10.1038/s41579-021-00577-w</identifier><identifier>PMID: 34172951</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/326/2522 ; 631/61/252/318 ; applied microbiology ; Bacteria - metabolism ; BASIC BIOLOGICAL SCIENCES ; Biodiesel fuels ; Biofuels ; Biofuels - economics ; Biomedical and Life Sciences ; Carbon ; Carbon sources ; Climate change ; Emissions ; Ethanol ; Fossil fuels ; Genetic Engineering ; Genomics ; Infectious Diseases ; Infrastructure ; Learning algorithms ; Life Sciences ; Machine Learning ; Medical Microbiology ; Metabolic engineering ; Metabolic pathways ; Metabolism ; Metabolites ; Microbiology ; Microorganisms ; Parasitology ; Petroleum ; Review Article ; Substrates ; Toxicity ; Transportation ; Virology</subject><ispartof>Nature reviews. Microbiology, 2021-11, Vol.19 (11), p.701-715</ispartof><rights>Springer Nature Limited 2021</rights><rights>2021. Springer Nature Limited.</rights><rights>Springer Nature Limited 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-c564e54a3f35eab20e8c74f086e74fff130cfd03426fca4f3c465d0b1cf75db93</citedby><cites>FETCH-LOGICAL-c446t-c564e54a3f35eab20e8c74f086e74fff130cfd03426fca4f3c465d0b1cf75db93</cites><orcidid>0000-0002-6513-7425 ; 0000-0002-4556-9685 ; 0000000245569685 ; 0000000265137425</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27898,27899</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34172951$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/servlets/purl/1902663$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Keasling, Jay</creatorcontrib><creatorcontrib>Garcia Martin, Hector</creatorcontrib><creatorcontrib>Lee, Taek Soon</creatorcontrib><creatorcontrib>Mukhopadhyay, Aindrila</creatorcontrib><creatorcontrib>Singer, Steven W.</creatorcontrib><creatorcontrib>Sundstrom, Eric</creatorcontrib><creatorcontrib>Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)</creatorcontrib><title>Microbial production of advanced biofuels</title><title>Nature reviews. Microbiology</title><addtitle>Nat Rev Microbiol</addtitle><addtitle>Nat Rev Microbiol</addtitle><description>Concerns over climate change have necessitated a rethinking of our transportation infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced by engineered microorganisms that use a renewable carbon source. Two biofuels, ethanol and biodiesel, have made inroads in displacing petroleum-based fuels, but their uptake has been limited by the amounts that can be used in conventional engines and by their cost. Advanced biofuels that mimic petroleum-based fuels are not limited by the amounts that can be used in existing transportation infrastructure but have had limited uptake due to costs. In this Review, we discuss engineering metabolic pathways to produce advanced biofuels, challenges with substrate and product toxicity with regard to host microorganisms and methods to engineer tolerance, and the use of functional genomics and machine learning approaches to produce advanced biofuels and prospects for reducing their costs.
Biofuels produced by conversion of biomass by engineered microorganisms have the potential to replace fossil fuels and reduce carbon emissions. In this Review, Keasling and colleagues discuss engineering of metabolic pathways to produce advanced biofuels and approaches to reduce metabolite toxicity and cost and increase titre, rate and yield.</description><subject>631/326/2522</subject><subject>631/61/252/318</subject><subject>applied microbiology</subject><subject>Bacteria - metabolism</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Biofuels - economics</subject><subject>Biomedical and Life Sciences</subject><subject>Carbon</subject><subject>Carbon sources</subject><subject>Climate change</subject><subject>Emissions</subject><subject>Ethanol</subject><subject>Fossil fuels</subject><subject>Genetic Engineering</subject><subject>Genomics</subject><subject>Infectious Diseases</subject><subject>Infrastructure</subject><subject>Learning algorithms</subject><subject>Life Sciences</subject><subject>Machine Learning</subject><subject>Medical Microbiology</subject><subject>Metabolic engineering</subject><subject>Metabolic pathways</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Parasitology</subject><subject>Petroleum</subject><subject>Review Article</subject><subject>Substrates</subject><subject>Toxicity</subject><subject>Transportation</subject><subject>Virology</subject><issn>1740-1526</issn><issn>1740-1534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAQhi0EoqXwBxhQBBND4PydjKjiSypigdlyHBtStXGxk1b8ewwpZWM6S_fce-cHoVMMVxhocR0Z5rLMgeAcgEuZb_bQGEsGOeaU7e_eRIzQUYxzAMK5JIdoRBmWpOR4jC6fGhN81ehFtgq-7k3X-DbzLtP1WrfG1lnVeNfbRTxGB04voj3Z1gl6vbt9mT7ks-f7x-nNLDeMiS43XDDLmaaOcqsrArYwkjkohE3FOUzBuBooI8IZzRw1TPAaKmyc5HVV0gk6H3J97BoVTdNZ825821rTKVwCEYIm6GKA0tEfvY2dmvs-tOkuRXgBBZElZokiA5W-GGOwTq1Cs9ThU2FQ3wrVoFAlhepHodqkobNtdF8tbb0b-XWWADoAMbXaNxv-dv8T-wVAdXtT</recordid><startdate>20211101</startdate><enddate>20211101</enddate><creator>Keasling, Jay</creator><creator>Garcia Martin, Hector</creator><creator>Lee, Taek Soon</creator><creator>Mukhopadhyay, Aindrila</creator><creator>Singer, Steven W.</creator><creator>Sundstrom, Eric</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><general>Springer Nature</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PCBAR</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>OIOZB</scope><scope>OTOTI</scope><orcidid>https://orcid.org/0000-0002-6513-7425</orcidid><orcidid>https://orcid.org/0000-0002-4556-9685</orcidid><orcidid>https://orcid.org/0000000245569685</orcidid><orcidid>https://orcid.org/0000000265137425</orcidid></search><sort><creationdate>20211101</creationdate><title>Microbial production of advanced biofuels</title><author>Keasling, Jay ; Garcia Martin, Hector ; Lee, Taek Soon ; Mukhopadhyay, Aindrila ; Singer, Steven W. ; Sundstrom, Eric</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-c564e54a3f35eab20e8c74f086e74fff130cfd03426fca4f3c465d0b1cf75db93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>631/326/2522</topic><topic>631/61/252/318</topic><topic>applied microbiology</topic><topic>Bacteria - metabolism</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biodiesel fuels</topic><topic>Biofuels</topic><topic>Biofuels - economics</topic><topic>Biomedical and Life Sciences</topic><topic>Carbon</topic><topic>Carbon sources</topic><topic>Climate change</topic><topic>Emissions</topic><topic>Ethanol</topic><topic>Fossil fuels</topic><topic>Genetic Engineering</topic><topic>Genomics</topic><topic>Infectious Diseases</topic><topic>Infrastructure</topic><topic>Learning algorithms</topic><topic>Life Sciences</topic><topic>Machine Learning</topic><topic>Medical Microbiology</topic><topic>Metabolic engineering</topic><topic>Metabolic pathways</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Parasitology</topic><topic>Petroleum</topic><topic>Review Article</topic><topic>Substrates</topic><topic>Toxicity</topic><topic>Transportation</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Keasling, Jay</creatorcontrib><creatorcontrib>Garcia Martin, Hector</creatorcontrib><creatorcontrib>Lee, Taek Soon</creatorcontrib><creatorcontrib>Mukhopadhyay, Aindrila</creatorcontrib><creatorcontrib>Singer, Steven W.</creatorcontrib><creatorcontrib>Sundstrom, Eric</creatorcontrib><creatorcontrib>Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database (ProQuest)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>OSTI.GOV - Hybrid</collection><collection>OSTI.GOV</collection><jtitle>Nature reviews. Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keasling, Jay</au><au>Garcia Martin, Hector</au><au>Lee, Taek Soon</au><au>Mukhopadhyay, Aindrila</au><au>Singer, Steven W.</au><au>Sundstrom, Eric</au><aucorp>Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbial production of advanced biofuels</atitle><jtitle>Nature reviews. Microbiology</jtitle><stitle>Nat Rev Microbiol</stitle><addtitle>Nat Rev Microbiol</addtitle><date>2021-11-01</date><risdate>2021</risdate><volume>19</volume><issue>11</issue><spage>701</spage><epage>715</epage><pages>701-715</pages><issn>1740-1526</issn><eissn>1740-1534</eissn><abstract>Concerns over climate change have necessitated a rethinking of our transportation infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced by engineered microorganisms that use a renewable carbon source. Two biofuels, ethanol and biodiesel, have made inroads in displacing petroleum-based fuels, but their uptake has been limited by the amounts that can be used in conventional engines and by their cost. Advanced biofuels that mimic petroleum-based fuels are not limited by the amounts that can be used in existing transportation infrastructure but have had limited uptake due to costs. In this Review, we discuss engineering metabolic pathways to produce advanced biofuels, challenges with substrate and product toxicity with regard to host microorganisms and methods to engineer tolerance, and the use of functional genomics and machine learning approaches to produce advanced biofuels and prospects for reducing their costs.
Biofuels produced by conversion of biomass by engineered microorganisms have the potential to replace fossil fuels and reduce carbon emissions. In this Review, Keasling and colleagues discuss engineering of metabolic pathways to produce advanced biofuels and approaches to reduce metabolite toxicity and cost and increase titre, rate and yield.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>34172951</pmid><doi>10.1038/s41579-021-00577-w</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-6513-7425</orcidid><orcidid>https://orcid.org/0000-0002-4556-9685</orcidid><orcidid>https://orcid.org/0000000245569685</orcidid><orcidid>https://orcid.org/0000000265137425</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1740-1526 |
| ispartof | Nature reviews. Microbiology, 2021-11, Vol.19 (11), p.701-715 |
| issn | 1740-1526 1740-1534 |
| language | eng |
| recordid | cdi_osti_scitechconnect_1902663 |
| source | North East Research Libraries Nature Academic Titles |
| subjects | 631/326/2522 631/61/252/318 applied microbiology Bacteria - metabolism BASIC BIOLOGICAL SCIENCES Biodiesel fuels Biofuels Biofuels - economics Biomedical and Life Sciences Carbon Carbon sources Climate change Emissions Ethanol Fossil fuels Genetic Engineering Genomics Infectious Diseases Infrastructure Learning algorithms Life Sciences Machine Learning Medical Microbiology Metabolic engineering Metabolic pathways Metabolism Metabolites Microbiology Microorganisms Parasitology Petroleum Review Article Substrates Toxicity Transportation Virology |
| title | Microbial production of advanced biofuels |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-27T00%3A44%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Microbial%20production%20of%20advanced%20biofuels&rft.jtitle=Nature%20reviews.%20Microbiology&rft.au=Keasling,%20Jay&rft.aucorp=Lawrence%20Berkeley%20National%20Laboratory%20(LBNL),%20Berkeley,%20CA%20(United%20States)&rft.date=2021-11-01&rft.volume=19&rft.issue=11&rft.spage=701&rft.epage=715&rft.pages=701-715&rft.issn=1740-1526&rft.eissn=1740-1534&rft_id=info:doi/10.1038/s41579-021-00577-w&rft_dat=%3Cproquest_osti_%3E2580827914%3C/proquest_osti_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c446t-c564e54a3f35eab20e8c74f086e74fff130cfd03426fca4f3c465d0b1cf75db93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2580827914&rft_id=info:pmid/34172951&rfr_iscdi=true |