Cysteine Mutants of the Major Facilitator Superfamily-Type Transporter CcoA Provide Insight into Copper Import

Copper (Cu) is a redox-active micronutrient that is both essential and toxic. Its cellular homeostasis is critical for supporting cuproprotein maturation while avoiding excessive oxidative stress. The Cu importer CcoA is the prototype of the widespread CalT subfamily of the MFS-type transporters. He...

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Published in:mBio 2021-08, Vol.12 (4)
Main Authors: Khalfaoui-Hassani, Bahia, Trasnea, Petru-Iulian, Steimle, Stefan, Koch, Hans-Georg, Daldal, Fevzi, Storz, Gisela
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Trasnea, Petru-Iulian
Steimle, Stefan
Koch, Hans-Georg
Daldal, Fevzi
Storz, Gisela
description Copper (Cu) is a redox-active micronutrient that is both essential and toxic. Its cellular homeostasis is critical for supporting cuproprotein maturation while avoiding excessive oxidative stress. The Cu importer CcoA is the prototype of the widespread CalT subfamily of the MFS-type transporters. Hence, understanding its molecular mechanism of function is significant. Here, we show that CcoA undergoes a thiol:disulfide oxidoreduction cycle, which is important for its Cu import activity.
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title Cysteine Mutants of the Major Facilitator Superfamily-Type Transporter CcoA Provide Insight into Copper Import
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