Sequence-Controlled Secondary Structures and Stimuli Responsiveness of Bioinspired Polyampholytes

A comprehensive study focusing on the influence of the sequence charge pattern on the secondary structure preferences of annealed polyampholytes and their responsiveness to external stimuli is presented. Two sequences are designed composed entirely of ionizable amino acids (charge fraction, f = 1) a...

Full description

Saved in:
Bibliographic Details
Published in:Biomacromolecules 2022-09, Vol.23 (9), p.3798-3809
Main Authors: Dinic, Jelena, Schnorenberg, Mathew R., Tirrell, Matthew V.
Format: Article
Language:English
Subjects:
BASIC BIOLOGICAL SCIENCES
helical structure
ion pairs
Peptides - chemistry
polyampholytes
Protein Structure, Secondary
secondary structure
sequence charge pattern
Solvents
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A comprehensive study focusing on the influence of the sequence charge pattern on the secondary structure preferences of annealed polyampholytes and their responsiveness to external stimuli is presented. Two sequences are designed composed entirely of ionizable amino acids (charge fraction, f = 1) and an equal number of positive and negative charges (f + = f – = 0.5) with distinct charge patterns consisting of lysine and glutamic acid monomers. The study reveals that the sequence charge pattern has a significant influence on the secondary structure preferences of polyampholytes at physiological pH. Furthermore, it shows that external stimuli such as pH, ionic strength, and solvent dielectric constant can be used to modulate the secondary structure of the two studied sequences. The observed secondary structure transformations for the two sequences are also substantially different from those determined for uniformly charged homo-polypeptides under matching conditions.
ISSN:1525-7797
1526-4602
DOI:10.1021/acs.biomac.2c00666