Characterization and biofouling potential analysis of two cyanobacterial strains isolated from Cape Verde and Morocco

• Published in: FEMS microbiology ecology 2023-02, Vol.99 (3)
• Main Authors: Romeu, Maria J, Morais, João, Gomes, Luciana C, Silva, Raquel, Vasconcelos, Vítor, Mergulhão, Filipe J M
• Format: Article
• Language: English
• Subjects: Biofilms; Biofouling; Cabo Verde; Chlorophyll; Chlorophyll A; Confocal microscopy; Cyanobacteria; Cyanobacteria - genetics; Imaging techniques; Morocco; Perspex; Phylogeny; Polymethyl Methacrylate; Qualitative analysis; Scanning microscopy
• ISSN: 1574-6941; 0168-6496; 1574-6941
• DOI: 10.1093/femsec/fiad004

Abstract Cyanobacteria are new sources of value-added compounds but also ubiquitous and harmful microfoulers on marine biofouling. In this work, the isolation and identification of two cyanobacterial strains isolated from Cape Verde and Morocco, as well as their biofilm-forming ability on glass and Perspex under controlled hydrodynamic conditions, were performed. Phylogenetic analysis revealed that cyanobacterial strains isolated belong to Leptothoe and Jaaginema genera (Leptothoe sp. LEGE 181153 and Jaaginema sp. LEGE 191154). From quantitative and qualitative data of wet weight, chlorophyll a content and biofilm thickness obtained by optical coherence tomography, no significant differences were found in biofilms developed by the same cyanobacterial strain on different surfaces (glass and Perspex). However, the biofilm-forming potential of Leptothoe sp. LEGE 181153 proved to be higher compared with Jaaginema sp. LEGE 191154, particularly at the maturation stage of biofilm development. Three-dimensional biofilm images obtained from confocal laser scanning microscopy showed different patterns between both cyanobacterial strains and also among the two surfaces. Because standard methodologies to evaluate cyanobacterial biofilm formation, as well as two different optical imaging techniques, were used, this work also highlights the possibility of integrating different techniques to evaluate a complex phenomenon like cyanobacterial biofilm development. This work assesses the isolation, identification and characterization of two cyanobacterial strains isolated from Morocco and Cape Verde, as well as their biofilm-forming ability at two different surfaces, in a long-term assay under controlled hydrodynamic conditions.