Effects of testosterone on mixed-muscle protein synthesis and proteome dynamics during energy deficit

Effects of testosterone on integrated muscle protein metabolism and muscle mass during energy deficit are undetermined. The objective was to determine the effects of testosterone on mixed-muscle protein synthesis (MPS), proteome-wide fractional synthesis rates (FSR), and skeletal muscle mass during...

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Published in:The journal of clinical endocrinology and metabolism 2022-08, Vol.107 (8), p.e3254-e3263
Main Authors: Howard, Emily E, Shankaran, Mahalakshmi, Evans, Willian J, Berryman, Claire E, Margolis, Lee M, Lieberman, Harris R, Karl, J Philip, Young, Andrew J, Montano, Monty A, Matthews, Marcy D, Bizieff, Alec, Nyangao, Edna, Mohammed, Hussein, Harris, Melissa N, Hellerstein, Marc K, Rood, Jennifer C, Pasiakos, Stefan M
Format: Article
Language:English
Subjects:
Analysis
Creatine
Endocrinology & Metabolism
Heavy water
Liquid chromatography
Medical research
Medicine, Experimental
Muscles
Oxandrolone
Protein biosynthesis
Testosterone
Weight loss maintenance
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Summary:Effects of testosterone on integrated muscle protein metabolism and muscle mass during energy deficit are undetermined. The objective was to determine the effects of testosterone on mixed-muscle protein synthesis (MPS), proteome-wide fractional synthesis rates (FSR), and skeletal muscle mass during energy deficit. This was a randomized, double-blind, placebo-controlled trial (ClinicalTrials.gov, NCT02734238). The study was conducted at Pennington Biomedical Research Center. Fifty healthy men. The study consisted of 14 days of weight maintenance, followed by a 28-day 55% energy deficit with 200 mg testosterone enanthate (TEST, n=24) or placebo (PLA, n=26) weekly, and up to 42 days of ad libitum recovery feeding. Mixed-MPS and proteome-wide FSR before (Pre), during (Mid) and after (Post) the energy deficit were determined using heavy water (days 1-42) and muscle biopsies. Muscle mass was determined using the D3-Creatine dilution method. Mixed-MPS was lower than Pre at Mid and Post (P
ISSN:0021-972X
1945-7197
DOI:10.1210/clinem/dgac295