Molecular dissection of the role of histidine in nickel hyperaccumulation in Thalspi goesingense (Halacsy)

To understand the role of free histidine (His) in Ni hyperaccumulation in Thlaspi goesingense, the authors investigated the regulation of His biosynthesis at both the molecular and biochemical levels. Three T. goesingense cDNAs encoding the following His biosynthetic enzymes, ATP phosphoribosyltrans...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1999-12, Vol.121 (4)
Main Authors: Persans, M.W., Yan, X., Patnoe, J.M.M.L., Kraemer, U., Salt, D.E.
Format: Article
Language:English
Subjects:
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
BIOLOGY AND MEDICINE, APPLIED STUDIES
BIOSYNTHESIS
ENVIRONMENTAL SCIENCES
GENE REGULATION
HISTIDINE
HYDRO-LYASES
LAND POLLUTION
NICKEL
OXIDOREDUCTASES
TRANSFERASES
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Summary:To understand the role of free histidine (His) in Ni hyperaccumulation in Thlaspi goesingense, the authors investigated the regulation of His biosynthesis at both the molecular and biochemical levels. Three T. goesingense cDNAs encoding the following His biosynthetic enzymes, ATP phosphoribosyltransferase, imidazoleglycerol phosphate dehydratase, and histidinol dehydrogenase, were isolated by functional complementation of Escherichia coli His autotrophs. Northern analysis of THJG1, THD1, and THB1 gene expression revealed that each gene is expressed in both roots and shoots, but at the concentrations and dosage times of Ni treatment used in this study, these genes failed to show any regulation by Ni. The authors were also unable to observe any increases in the concentration of free His in root, shoot, or xylem sap of T. goesingense in response to Ni exposure. X-ray absorption spectroscopy of root and shoot tissue from T. goesingense and the non-accumulator species Thlaspi reverse revealed no major differences in the coordination of Ni by His in these tissues. They therefore conclude that the Ni hyperaccumulation phenotype in T. goesingense is not determined by the overproduction of His in response to Ni.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.121.4.1117