SIBYLS - a SAXS and Protein Crystallography Beamline at the ALS

• Main Authors: Trame, C, MacDowell, A A, Celestre, R S, Padmore, H A, Cambie, D, Domning, E E, Duarte, R M, Kelez, N, Plate, D W, Holton, J M, Frankel, K, Tsutakawa, S, Tsuruta, H, Tainer, J A, Cooper, P K
• Format: Conference Proceeding
• Language: English
• Subjects: ADVANCED LIGHT SOURCE; APERTURES; BEAM OPTICS; CRYSTALLOGRAPHY; CRYSTALS; DIPOLES; ELECTRON BEAMS; GEV RANGE 01-10; HARD X RADIATION; PARTICLE ACCELERATORS; PROTEINS; RESOLUTION; SILICON; SMALL ANGLE SCATTERING; SOLUTIONS; SYNCHROTRON RADIATION; X-RAY DIFFRACTION; X-RAY SOURCES
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/1.1757844

The new Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the Advanced Light Source will be dedicated to Macromolecular Crystallography (PX) and Small Angle X-ray Scattering (SAXS). SAXS will provide structural information of macromolecules in solutions and will complement high resolution PX studies on the same systems but in a crystalline state. The x-ray source is one of the 5 Tesla superbend dipoles recently installed at the ALS that allows for a hard x-ray program to be developed on the relatively low energy Advanced Light Source (ALS) ring (1.9 GeV). The beamline is equipped with fast interchangeable monochromator elements, consisting of either a pair of single Si(111) crystals for crystallography, or a pair of multilayers for the SAXS mode data collection (E/E~1/110). Flux rates with Si(111) crystals for PX are measured as 2X1011 hv/sec through a 100mum pinhole at 12.4KeV. For SAXS the flux is up to 3X1013photons/sec at 10KeV with all apertures open when using the multilayer monochromator elements. The performance characteristics of this unique beamline will be described.