Photodynamic effects of a novel pterin derivative on a pancreatic cancer cell line

6-Formylpterin (6FP) has the potential to produce singlet oxygen ( 1O 2) under UV-A radiation. In order to apply this potential to anti-cancer photodynamic therapy (PDT), we prepared a novel variant of 6FP, 2-( N, N-dimethylaminomethyleneamino)-6-formyl-3-pivaloylpteridine-4-one (6FP-tBu-DMF), and e...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-08, Vol.333 (3), p.763-767
Main Authors: Yamada, Hiroko, Arai, Toshiyuki, Endo, Nobuyuki, Yamashita, Kouhei, Nonogawa, Mitsuru, Makino, Keisuke, Fukuda, Kazuhiko, Sasada, Masataka, Uchiyama, Takashi
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
APOPTOSIS
AQUEOUS SOLUTIONS
Cell Line, Tumor
Cell permeability
DRUGS
Electron Spin Resonance Spectroscopy
Humans
Microscopy, Confocal
NEOPLASMS
OXYGEN
Panc-1 cells
PANCREAS
Pancreatic Neoplasms - drug therapy
Pancreatic Neoplasms - pathology
PERMEABILITY
Photochemotherapy
Photodynamic effects
Pteridines - therapeutic use
Pterin derivatives
Singlet oxygen
THERAPY
UPTAKE
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Summary:6-Formylpterin (6FP) has the potential to produce singlet oxygen ( 1O 2) under UV-A radiation. In order to apply this potential to anti-cancer photodynamic therapy (PDT), we prepared a novel variant of 6FP, 2-( N, N-dimethylaminomethyleneamino)-6-formyl-3-pivaloylpteridine-4-one (6FP-tBu-DMF), and examined its photodynamic effects on a pancreatic cancer cell line, Panc-1 cells. The study using laser scanning confocal microscopy showed that the drug uptake, the 1O 2 generation, and cell death were observed in the 6FP-tBu-DMF-treated cells, while these phenomena were not observed in the 6FP-treated cells. The MTT assay also showed the decrease in cell viability only in the 6FP-tBu-DMF-treated cells. Since 6FP and 6FP-tBu-DMF generate 1O 2 to the same extent under UV-A radiation in aqueous solutions, these results indicated that the differences in the photodynamic effects between 6FP and 6FP-tBu-DMF were entirely attributed to the differences in the cell permeability between them. The development of cell permeable pterin derivatives has the potential for application in PDT.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.05.185