Plastid DNA polymerases from higher plants, Arabidopsis thaliana

Previously, we described a novel DNA polymerase, designated as OsPolI-like, from rice. The OsPolI-like showed a high degree of sequence homology with the DNA polymerase I of cyanobacteria and was localized in the plastid. Here, we describe two PolI-like polymerases, designated as AtPolI-like A and A...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-08, Vol.334 (1), p.43-50
Main Authors: Mori, Yoko, Kimura, Seisuke, Saotome, Ai, Kasai, Nobuyuki, Sakaguchi, Norihiro, Uchiyama, Yukinobu, Ishibashi, Toyotaka, Yamamoto, Taichi, Chiku, Hiroyuki, Sakaguchi, Kengo
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Amino Acid Sequence
ANIMALS
ARABIDOPSIS
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis thaliana
AtPolI-like A
AtPolI-like B
Chloroplast
CHLOROPLASTS
CYANOBACTERIA
DNA
DNA DAMAGES
DNA Polymerase I - chemistry
DNA Polymerase I - genetics
DNA Polymerase I - metabolism
DNA POLYMERASES
DNA REPAIR
DNA REPLICATION
HYDROGEN PEROXIDE
IN-SITU HYBRIDIZATION
MERISTEMS
Molecular Sequence Data
Oryza sativa
OXIDATION
Plant Structures - genetics
Plant Structures - metabolism
Plastid
Plastids - genetics
Plastids - metabolism
RICE
Sequence Homology, Amino Acid
Tissue Distribution
YEASTS
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Summary:Previously, we described a novel DNA polymerase, designated as OsPolI-like, from rice. The OsPolI-like showed a high degree of sequence homology with the DNA polymerase I of cyanobacteria and was localized in the plastid. Here, we describe two PolI-like polymerases, designated as AtPolI-like A and AtPolI-like B, from Arabidopsis thaliana. In situ hybridization analysis demonstrated expression of both mRNAs in proliferating tissues such as the shoot apical meristem. Analysis of the localizations of GFP fusion proteins showed that AtPolI-like A and AtPolI-like B were localized to plastids. AtPolI-like B expression could be induced by exposure to the mutagen H 2O 2. These results suggested that AtPolI-like B has a role in the repair of oxidation-induced DNA damage. Our data indicate that higher plants possess two plastid DNA polymerases that are not found in animals and yeasts.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.06.052