Deinococcus radiodurans strain R1 contains N6-methyladenine in its genome

Methylation of DNA is known to be involved in DNA repair mechanisms in bacteria. Deinococcus radiodurans strain R1 on exposure to high radiation undergoes significant DNA damage, which is repaired without mutations. However, the presence of modified nucleotides has not been reported in its genome. W...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-09, Vol.335 (2), p.412-416
Main Authors: Prasad, Bhaskar Jyoti, Sabnis, Ketaki, Deobagkar, Deepti D., Deobagkar, Dileep N.
Format: Article
Language:English
Subjects:
Adenine - analogs & derivatives
Adenine - chemistry
Adenine - metabolism
ADENINES
Anti-N6-methyladenine antibody
ANTIBODIES
BACTERIA
BIOLOGICAL RADIATION EFFECTS
Cell-Free System
Chromatography - methods
Deinococcus - genetics
Deinococcus radiodurans
DNA
DNA - chemistry
DNA Damage
DNA DAMAGES
DNA Methylation
DNA REPAIR
DNA, Bacterial
Genome, Bacterial
Immunochemical staining
Immunochemistry
IN VITRO
In vitro methylation
IRRADIATION
METHYLATION
MUTATIONS
NUCLEOTIDES
Plant Growth Regulators - genetics
Polymerase Chain Reaction
Post-irradiation recovery
Radiation
RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism
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Description
Summary:Methylation of DNA is known to be involved in DNA repair mechanisms in bacteria. Deinococcus radiodurans strain R1 on exposure to high radiation undergoes significant DNA damage, which is repaired without mutations. However, the presence of modified nucleotides has not been reported in its genome. We report here the detection of N6-methyladenine in the genome of D. radiodurans strain R1 using immunochemical techniques. This N6-methyladenine is not a part of GATC restriction-modification system. D. radiodurans cell extract also exhibited a DNA adenine methyltransferase activity which was reduced in the early post-irradiation recovery phase.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.07.099