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Deinococcus radiodurans strain R1 contains N6-methyladenine in its genome
Methylation of DNA is known to be involved in DNA repair mechanisms in bacteria. Deinococcus radiodurans strain R1 on exposure to high radiation undergoes significant DNA damage, which is repaired without mutations. However, the presence of modified nucleotides has not been reported in its genome. W...
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Published in: | Biochemical and biophysical research communications 2005-09, Vol.335 (2), p.412-416 |
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container_title | Biochemical and biophysical research communications |
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creator | Prasad, Bhaskar Jyoti Sabnis, Ketaki Deobagkar, Deepti D. Deobagkar, Dileep N. |
description | Methylation of DNA is known to be involved in DNA repair mechanisms in bacteria.
Deinococcus radiodurans strain R1 on exposure to high radiation undergoes significant DNA damage, which is repaired without mutations. However, the presence of modified nucleotides has not been reported in its genome. We report here the detection of N6-methyladenine in the genome of
D. radiodurans strain R1 using immunochemical techniques. This N6-methyladenine is not a part of GATC restriction-modification system.
D. radiodurans cell extract also exhibited a DNA adenine methyltransferase activity which was reduced in the early post-irradiation recovery phase. |
doi_str_mv | 10.1016/j.bbrc.2005.07.099 |
format | article |
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Deinococcus radiodurans strain R1 on exposure to high radiation undergoes significant DNA damage, which is repaired without mutations. However, the presence of modified nucleotides has not been reported in its genome. We report here the detection of N6-methyladenine in the genome of
D. radiodurans strain R1 using immunochemical techniques. This N6-methyladenine is not a part of GATC restriction-modification system.
D. radiodurans cell extract also exhibited a DNA adenine methyltransferase activity which was reduced in the early post-irradiation recovery phase.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2005.07.099</identifier><identifier>PMID: 16084831</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenine - analogs & derivatives ; Adenine - chemistry ; Adenine - metabolism ; ADENINES ; Anti-N6-methyladenine antibody ; ANTIBODIES ; BACTERIA ; BIOLOGICAL RADIATION EFFECTS ; Cell-Free System ; Chromatography - methods ; Deinococcus - genetics ; Deinococcus radiodurans ; DNA ; DNA - chemistry ; DNA Damage ; DNA DAMAGES ; DNA Methylation ; DNA REPAIR ; DNA, Bacterial ; Genome, Bacterial ; Immunochemical staining ; Immunochemistry ; IN VITRO ; In vitro methylation ; IRRADIATION ; METHYLATION ; MUTATIONS ; NUCLEOTIDES ; Plant Growth Regulators - genetics ; Polymerase Chain Reaction ; Post-irradiation recovery ; Radiation ; RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS ; Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2005-09, Vol.335 (2), p.412-416</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-59b797a8660e0ef95067186b87ae7fbbeb44181a84d81bf4235adde9844f91d33</citedby><cites>FETCH-LOGICAL-c413t-59b797a8660e0ef95067186b87ae7fbbeb44181a84d81bf4235adde9844f91d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16084831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20710978$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Prasad, Bhaskar Jyoti</creatorcontrib><creatorcontrib>Sabnis, Ketaki</creatorcontrib><creatorcontrib>Deobagkar, Deepti D.</creatorcontrib><creatorcontrib>Deobagkar, Dileep N.</creatorcontrib><title>Deinococcus radiodurans strain R1 contains N6-methyladenine in its genome</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Methylation of DNA is known to be involved in DNA repair mechanisms in bacteria.
Deinococcus radiodurans strain R1 on exposure to high radiation undergoes significant DNA damage, which is repaired without mutations. However, the presence of modified nucleotides has not been reported in its genome. We report here the detection of N6-methyladenine in the genome of
D. radiodurans strain R1 using immunochemical techniques. This N6-methyladenine is not a part of GATC restriction-modification system.
D. radiodurans cell extract also exhibited a DNA adenine methyltransferase activity which was reduced in the early post-irradiation recovery phase.</description><subject>Adenine - analogs & derivatives</subject><subject>Adenine - chemistry</subject><subject>Adenine - metabolism</subject><subject>ADENINES</subject><subject>Anti-N6-methyladenine antibody</subject><subject>ANTIBODIES</subject><subject>BACTERIA</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>Cell-Free System</subject><subject>Chromatography - methods</subject><subject>Deinococcus - genetics</subject><subject>Deinococcus radiodurans</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>DNA Damage</subject><subject>DNA DAMAGES</subject><subject>DNA Methylation</subject><subject>DNA REPAIR</subject><subject>DNA, Bacterial</subject><subject>Genome, Bacterial</subject><subject>Immunochemical staining</subject><subject>Immunochemistry</subject><subject>IN VITRO</subject><subject>In vitro methylation</subject><subject>IRRADIATION</subject><subject>METHYLATION</subject><subject>MUTATIONS</subject><subject>NUCLEOTIDES</subject><subject>Plant Growth Regulators - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Post-irradiation recovery</subject><subject>Radiation</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS</subject><subject>Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkU2LFDEQhoMo7rj6BzxIg-Ct26rudD7Ai6xfC4uCKHgL6aTazTCTrEm3sP_eNDPgTU8pyFMvVfUw9hyhQ0Dxet9NU3ZdDzB2IDvQ-gHbIWhoewT-kO0AQLS9xh8X7EkpewBELvRjdoECFFcD7tj1OwoxueTcWppsfUh-zTaWpizZhth8xcaluNSyNJ9Fe6Tl9v5gPcUQqan_YSnNT4rpSE_Zo9keCj07v5fs-4f3364-tTdfPl5fvb1pHcdhaUc9SS2tEgIIaNYjCIlKTEpakvM00cQ5KrSKe4XTzPthtN6TVpzPGv0wXLKXp9xUlmCKCwu52zpjJLeYHmQ9gFSVenWi7nL6tVJZzDEUR4eDjZTWYoTikksY_wuiHORYyQr2J9DlVEqm2dzlcLT53iCYzYfZm82H2XwYkKb6qE0vzunrdCT_t-UsoAJvTgDVk_0OlLeNKDryIW8L-RT-lf8HOleang</recordid><startdate>20050923</startdate><enddate>20050923</enddate><creator>Prasad, Bhaskar Jyoti</creator><creator>Sabnis, Ketaki</creator><creator>Deobagkar, Deepti D.</creator><creator>Deobagkar, Dileep N.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20050923</creationdate><title>Deinococcus radiodurans strain R1 contains N6-methyladenine in its genome</title><author>Prasad, Bhaskar Jyoti ; Sabnis, Ketaki ; Deobagkar, Deepti D. ; Deobagkar, Dileep N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-59b797a8660e0ef95067186b87ae7fbbeb44181a84d81bf4235adde9844f91d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adenine - analogs & derivatives</topic><topic>Adenine - chemistry</topic><topic>Adenine - metabolism</topic><topic>ADENINES</topic><topic>Anti-N6-methyladenine antibody</topic><topic>ANTIBODIES</topic><topic>BACTERIA</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>Cell-Free System</topic><topic>Chromatography - methods</topic><topic>Deinococcus - genetics</topic><topic>Deinococcus radiodurans</topic><topic>DNA</topic><topic>DNA - chemistry</topic><topic>DNA Damage</topic><topic>DNA DAMAGES</topic><topic>DNA Methylation</topic><topic>DNA REPAIR</topic><topic>DNA, Bacterial</topic><topic>Genome, Bacterial</topic><topic>Immunochemical staining</topic><topic>Immunochemistry</topic><topic>IN VITRO</topic><topic>In vitro methylation</topic><topic>IRRADIATION</topic><topic>METHYLATION</topic><topic>MUTATIONS</topic><topic>NUCLEOTIDES</topic><topic>Plant Growth Regulators - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Post-irradiation recovery</topic><topic>Radiation</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS</topic><topic>Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prasad, Bhaskar Jyoti</creatorcontrib><creatorcontrib>Sabnis, Ketaki</creatorcontrib><creatorcontrib>Deobagkar, Deepti D.</creatorcontrib><creatorcontrib>Deobagkar, Dileep N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prasad, Bhaskar Jyoti</au><au>Sabnis, Ketaki</au><au>Deobagkar, Deepti D.</au><au>Deobagkar, Dileep N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deinococcus radiodurans strain R1 contains N6-methyladenine in its genome</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2005-09-23</date><risdate>2005</risdate><volume>335</volume><issue>2</issue><spage>412</spage><epage>416</epage><pages>412-416</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Methylation of DNA is known to be involved in DNA repair mechanisms in bacteria.
Deinococcus radiodurans strain R1 on exposure to high radiation undergoes significant DNA damage, which is repaired without mutations. However, the presence of modified nucleotides has not been reported in its genome. We report here the detection of N6-methyladenine in the genome of
D. radiodurans strain R1 using immunochemical techniques. This N6-methyladenine is not a part of GATC restriction-modification system.
D. radiodurans cell extract also exhibited a DNA adenine methyltransferase activity which was reduced in the early post-irradiation recovery phase.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16084831</pmid><doi>10.1016/j.bbrc.2005.07.099</doi><tpages>5</tpages></addata></record> |
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subjects | Adenine - analogs & derivatives Adenine - chemistry Adenine - metabolism ADENINES Anti-N6-methyladenine antibody ANTIBODIES BACTERIA BIOLOGICAL RADIATION EFFECTS Cell-Free System Chromatography - methods Deinococcus - genetics Deinococcus radiodurans DNA DNA - chemistry DNA Damage DNA DAMAGES DNA Methylation DNA REPAIR DNA, Bacterial Genome, Bacterial Immunochemical staining Immunochemistry IN VITRO In vitro methylation IRRADIATION METHYLATION MUTATIONS NUCLEOTIDES Plant Growth Regulators - genetics Polymerase Chain Reaction Post-irradiation recovery Radiation RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism |
title | Deinococcus radiodurans strain R1 contains N6-methyladenine in its genome |
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