Detection and quantitation of benzo(a)pyrene-derived DNA adducts in mouse liver by liquid chromatography - tandem mass spectrometry: comparison with P-32-postlabeling

The polycyclic aromatic hydrocarbon, benzo(a)pyrene (B(a)P) is a proven animal carcinogen that is potentially carcinogenic to humans. B( a)P is an ubiquitous environmental pollutant and is also present in tobacco smoke, coal tar, automobile exhaust emissions, and charred food. A liquid chromatograph...

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Bibliographic Details
Published in:Chemical research in toxicology 2006-06, Vol.19 (6)
Main Authors: Singh, R., Gaskell, M., Le Pla, R.C., Kaur, B., Azim-Araghi, A., Roach, J., Koukouves, G., Souliotis, V.L., Kyrtopoulos, S.A., Farmer, P.B.
Format: Article
Language:English
Subjects:
01 COAL, LIGNITE, AND PEAT
BENZOPYRENE
DNA ADDUCTS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MASS SPECTROSCOPY
MICE
POLYCYCLIC AROMATIC HYDROCARBONS
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Summary:The polycyclic aromatic hydrocarbon, benzo(a)pyrene (B(a)P) is a proven animal carcinogen that is potentially carcinogenic to humans. B( a)P is an ubiquitous environmental pollutant and is also present in tobacco smoke, coal tar, automobile exhaust emissions, and charred food. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using electrospray ionization and selected reaction monitoring (SRM) has been developed for the detection of 10-(deoxyguanosin-N{sub 2}-yl)-7,8,9-trihydroxy-7,8,9,10- tetrahydrobenzo(a)pyrene (B(a)PDE-N{sub 2}dG) adducts formed in DNA following the metabolic activation of B(a)P to benzo(a) pyrene-7,8-dihydrodiol-9,10-epoxide (B(a)PDE).
ISSN:0893-228X
1520-5010
DOI:10.1021/tx060011r