Wnt-10b promotes differentiation of skin epithelial cells in vitro

To evaluate the role of Wnt-10b in epithelial differentiation, we investigated the effects of Wnt-10b on adult mouse-derived primary skin epithelial cells (MPSEC). Recombinant Wnt-10b protein (rWnt-10b) was prepared using a gene engineering technique and MPSEC were cultured in its presence, which re...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-03, Vol.342 (1), p.28-35
Main Authors: Ouji, Yukiteru, Yoshikawa, Masahide, Shiroi, Akira, Ishizaka, Shigeaki
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ADULTS
Animals
Cell Differentiation
CELL PROLIFERATION
Cell Proliferation - drug effects
Cells, Cultured
Cercopithecus aethiops
Differentiation
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Female
Gene Expression Regulation - drug effects
GENES
GENETIC ENGINEERING
HAIR
Hair Follicle - cytology
Hair Follicle - metabolism
HAIR FOLLICLES
IN VITRO
KERATIN
MICE
Mice, Inbred C3H
MORPHOLOGICAL CHANGES
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins - pharmacology
Recombinant Proteins - pharmacology
RNA, Messenger - genetics
Signal Transduction
Skin - cytology
Skin - drug effects
Skin - metabolism
Skin epithelial cell
Wnt Proteins - genetics
Wnt Proteins - metabolism
Wnt Proteins - pharmacology
Wnt-10b
β-Catenin
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Summary:To evaluate the role of Wnt-10b in epithelial differentiation, we investigated the effects of Wnt-10b on adult mouse-derived primary skin epithelial cells (MPSEC). Recombinant Wnt-10b protein (rWnt-10b) was prepared using a gene engineering technique and MPSEC were cultured in its presence, which resulted in morphological changes from cuboidal to spindle-shaped and inhibited their proliferation. Further, involvement of the canonical Wnt signal pathway was also observed. MPSEC treated with rWnt-10b showed characteristics of the hair shaft and inner root sheath of the hair follicle, in results of Ayoub Shklar staining and immunocytochemistry. Further, the cells expressed mRNA for differentiated epithelial cells, including keratin 1, keratin 2, loricrin, mHa5, and mHb5, in association with a decreased expression of the basal cell marker keratin 5. These results suggest that Wnt-10b promotes the differentiation of MPSEC.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.01.104