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Wnt-10b promotes differentiation of skin epithelial cells in vitro
To evaluate the role of Wnt-10b in epithelial differentiation, we investigated the effects of Wnt-10b on adult mouse-derived primary skin epithelial cells (MPSEC). Recombinant Wnt-10b protein (rWnt-10b) was prepared using a gene engineering technique and MPSEC were cultured in its presence, which re...
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Published in: | Biochemical and biophysical research communications 2006-03, Vol.342 (1), p.28-35 |
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description | To evaluate the role of Wnt-10b in epithelial differentiation, we investigated the effects of Wnt-10b on adult mouse-derived primary skin epithelial cells (MPSEC). Recombinant Wnt-10b protein (rWnt-10b) was prepared using a gene engineering technique and MPSEC were cultured in its presence, which resulted in morphological changes from cuboidal to spindle-shaped and inhibited their proliferation. Further, involvement of the canonical Wnt signal pathway was also observed. MPSEC treated with rWnt-10b showed characteristics of the hair shaft and inner root sheath of the hair follicle, in results of Ayoub Shklar staining and immunocytochemistry. Further, the cells expressed mRNA for differentiated epithelial cells, including keratin 1, keratin 2, loricrin, mHa5, and mHb5, in association with a decreased expression of the basal cell marker keratin 5. These results suggest that Wnt-10b promotes the differentiation of MPSEC. |
doi_str_mv | 10.1016/j.bbrc.2006.01.104 |
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Recombinant Wnt-10b protein (rWnt-10b) was prepared using a gene engineering technique and MPSEC were cultured in its presence, which resulted in morphological changes from cuboidal to spindle-shaped and inhibited their proliferation. Further, involvement of the canonical Wnt signal pathway was also observed. MPSEC treated with rWnt-10b showed characteristics of the hair shaft and inner root sheath of the hair follicle, in results of Ayoub Shklar staining and immunocytochemistry. Further, the cells expressed mRNA for differentiated epithelial cells, including keratin 1, keratin 2, loricrin, mHa5, and mHb5, in association with a decreased expression of the basal cell marker keratin 5. 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Yoshikawa, Masahide ; Shiroi, Akira ; Ishizaka, Shigeaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-1dfb764a6d36033c5700b766aacdd939f213355eba541c9891420b19a7e66a6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>ADULTS</topic><topic>Animals</topic><topic>Cell Differentiation</topic><topic>CELL PROLIFERATION</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Cercopithecus aethiops</topic><topic>Differentiation</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation - drug effects</topic><topic>GENES</topic><topic>GENETIC ENGINEERING</topic><topic>HAIR</topic><topic>Hair Follicle - cytology</topic><topic>Hair Follicle - metabolism</topic><topic>HAIR FOLLICLES</topic><topic>IN VITRO</topic><topic>KERATIN</topic><topic>MICE</topic><topic>Mice, Inbred C3H</topic><topic>MORPHOLOGICAL CHANGES</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Proto-Oncogene Proteins - pharmacology</topic><topic>Recombinant Proteins - pharmacology</topic><topic>RNA, Messenger - genetics</topic><topic>Signal Transduction</topic><topic>Skin - cytology</topic><topic>Skin - drug effects</topic><topic>Skin - metabolism</topic><topic>Skin epithelial cell</topic><topic>Wnt Proteins - genetics</topic><topic>Wnt Proteins - metabolism</topic><topic>Wnt Proteins - pharmacology</topic><topic>Wnt-10b</topic><topic>β-Catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ouji, Yukiteru</creatorcontrib><creatorcontrib>Yoshikawa, Masahide</creatorcontrib><creatorcontrib>Shiroi, Akira</creatorcontrib><creatorcontrib>Ishizaka, Shigeaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ouji, Yukiteru</au><au>Yoshikawa, Masahide</au><au>Shiroi, Akira</au><au>Ishizaka, Shigeaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wnt-10b promotes differentiation of skin epithelial cells in vitro</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-03-31</date><risdate>2006</risdate><volume>342</volume><issue>1</issue><spage>28</spage><epage>35</epage><pages>28-35</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>To evaluate the role of Wnt-10b in epithelial differentiation, we investigated the effects of Wnt-10b on adult mouse-derived primary skin epithelial cells (MPSEC). Recombinant Wnt-10b protein (rWnt-10b) was prepared using a gene engineering technique and MPSEC were cultured in its presence, which resulted in morphological changes from cuboidal to spindle-shaped and inhibited their proliferation. Further, involvement of the canonical Wnt signal pathway was also observed. MPSEC treated with rWnt-10b showed characteristics of the hair shaft and inner root sheath of the hair follicle, in results of Ayoub Shklar staining and immunocytochemistry. Further, the cells expressed mRNA for differentiated epithelial cells, including keratin 1, keratin 2, loricrin, mHa5, and mHb5, in association with a decreased expression of the basal cell marker keratin 5. These results suggest that Wnt-10b promotes the differentiation of MPSEC.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16469292</pmid><doi>10.1016/j.bbrc.2006.01.104</doi><tpages>8</tpages></addata></record> |
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subjects | 60 APPLIED LIFE SCIENCES ADULTS Animals Cell Differentiation CELL PROLIFERATION Cell Proliferation - drug effects Cells, Cultured Cercopithecus aethiops Differentiation Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - metabolism Female Gene Expression Regulation - drug effects GENES GENETIC ENGINEERING HAIR Hair Follicle - cytology Hair Follicle - metabolism HAIR FOLLICLES IN VITRO KERATIN MICE Mice, Inbred C3H MORPHOLOGICAL CHANGES Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins - pharmacology Recombinant Proteins - pharmacology RNA, Messenger - genetics Signal Transduction Skin - cytology Skin - drug effects Skin - metabolism Skin epithelial cell Wnt Proteins - genetics Wnt Proteins - metabolism Wnt Proteins - pharmacology Wnt-10b β-Catenin |
title | Wnt-10b promotes differentiation of skin epithelial cells in vitro |
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