Effect of Nd{sup 3+} ion on carboxylation activity of ribulose-1,5-bisphosphate carboxylase/oxygenase of spinach

Neodymium (Nd), as a member of rare earth elements, proved to enhance the photosynthesis rate and organic substance accumulation of spinach through the increase in carboxylation activity of Rubisco. Although the oxygenase activity of spinach Rubisco was slightly changed with the Nd{sup 3+} treatment...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-03, Vol.342 (1)
Main Authors: Liu Chao, Hong Fashui, Wu Kang, Ma, Hong-bing, Zhang Xueguang, Hong Chengjiao, Wu Cheng, Gao Fengqing, Yang Fan, Zheng Lei, Wang Xuefeng, Liu Tao, Xie Yaning, Xu Jianhua, Li Zhongrui
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ABSORPTION SPECTROSCOPY
AMINO ACIDS
BOND LENGTHS
CARBOXYLASE
ELECTROPHORESIS
FINE STRUCTURE
GELS
NEODYMIUM
NEODYMIUM IONS
OXYGEN
PHOTOSYNTHESIS
RIBULOSE
SPINACH
SULFUR
X-RAY SPECTROSCOPY
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Summary:Neodymium (Nd), as a member of rare earth elements, proved to enhance the photosynthesis rate and organic substance accumulation of spinach through the increase in carboxylation activity of Rubisco. Although the oxygenase activity of spinach Rubisco was slightly changed with the Nd{sup 3+} treatment, the specific factor of Rubisco was greatly increased. It was partially due to the promotion of Rubisco activase (R-A) activity but mainly to the formation of Rubisco-Rubisco activase super-complex, a heavier molecular mass protein (about 1200 kD) comprising both Rubisco and Rubisco activase. This super-complex was found during the extraction procedure of Rubisco by the gel electrophoresis and Western-blot studies. The formation of Rubisco-R-A super-complex suggested that the secondary structure of the protein purified from the Nd{sup 3+}-treated spinach was different from that of the control. Extended X-ray absorption fine structure study of the 'Rubisco' purified from the Nd{sup 3+}-treated spinach revealed that Nd was bound with four oxygen atoms and two sulfur atoms of amino acid residues at the Nd-O and Nd-S bond lengths of 2.46 and 2.89 A, respectively.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.01.105