Nicotine prevents the apoptosis induced by menadione in human lung cancer cells

Approximately 50% of long-term cigarette smokers die prematurely from the adverse effects of smoking, including on lung cancer and other illnesses. Nicotine is a main component in tobacco and has been implicated as a potential factor in the pathogenesis of human lung cancer. However, the mechanism o...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-04, Vol.342 (3), p.928-934
Main Authors: Zhang, Tao, Lu, Heng, Shang, Xuan, Tian, Yihao, Zheng, Congyi, Wang, Shiwen, Cheng, Hanhua, Zhou, Rongjia
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
APOPTOSIS
Apoptosis - drug effects
CELL PROLIFERATION
Cell Proliferation - drug effects
CHEMOTHERAPY
Humans
Lung cancer
Lung Neoplasms - pathology
LUNGS
NEOPLASMS
NF-kappa B - genetics
NF-κB
NICOTINE
Nicotine - pharmacology
PATHOGENESIS
PATIENTS
RNA Interference
RNA, Messenger - genetics
RNA, Messenger - metabolism
TOBACCO
Vitamin K 3 - pharmacology
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Summary:Approximately 50% of long-term cigarette smokers die prematurely from the adverse effects of smoking, including on lung cancer and other illnesses. Nicotine is a main component in tobacco and has been implicated as a potential factor in the pathogenesis of human lung cancer. However, the mechanism of nicotine action in the development of lung cancer remains largely unknown. In the present study, we designed a nicotine-apoptosis system, by pre-treatment of nicotine making lung cancer cell A549 to be in a physiological nicotine environment, and observed that nicotine promoted cell proliferation and prevented the menadione-induced apoptosis, and exerts its role of anti-apoptosis by shift of apoptotic stage induced by menadione from late apoptotic stage to early apoptotic stage, in which NF-κB was up-regulated. Interference analysis of NF-κB in A549 cells showed that knock down of NF-κB resulted in apoptosis promotion and counteracted the protective effect of nicotine. The findings suggest that nicotine has potential effect in lung cancer genesis, especially in patients with undetectable early tumor development and development of specific NF-κB inhibitors would represent a potentially exciting new pharmacotherapy for tobacco-related lung cancer.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.02.047