F F{sub 1}-ATPase as biosensor to detect single virus

F F{sub 1}-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody-antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F F{sub 1}-ATPase, which w...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-04, Vol.342 (4)
Main Authors: Liu, XiaoLong, The Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, Zhang, Yun, Yue, JiaChang, Jiang, PeiDong, Zhang, ZhenXi
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ANTIBODIES
ANTIGENS
ATP
ATP-ASE
BIOSYNTHESIS
FLUORESCENCE
INFLUENZA VIRUSES
MICROSCOPES
SIGNAL-TO-NOISE RATIO
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Summary:F F{sub 1}-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody-antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F F{sub 1}-ATPase, which was indicated by F1300, was directly observed by a fluorescence microscope. The results demonstrate that the biosensor loading of virus particles has remarkable signal-to-noise ratio (3.8:1) compared to its control at single molecular level, and will be convenient, quick, and even super-sensitive for detecting virus particles.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.02.103