Truncated form of VACM-1/cul-5 with an extended 3′ untranslated region stimulates cell growth via a MAPK-dependent pathway

We have sequenced a 4.9 kb clone (KLB22) which shares 99% sequence homology with the rabbit vasopressin-activated calcium mobilizing (VACM-1) protein. The 5′ terminus sequence of KLB22 cDNA (nucleotides 1–1961) is continuous and overlapping with nucleotides 1226–3186 of the VACM-1 cDNA sequence. The...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-05, Vol.343 (4), p.1086-1093
Main Authors: Sartor, Ashleigh, Kossoris, J.B., Wilcox, R., Shearer, R., Zeneberg, A.E., Zhao, P., Lazdins, I., Burnatowska-Hledin, Maria A.
Format: Article
Language:English
Subjects:
3' Untranslated Regions
60 APPLIED LIFE SCIENCES
Amino Acid Sequence
AMINO ACIDS
Animals
CALCIUM
Cell growth
Cell Nucleus - metabolism
CELL PROLIFERATION
Cercopithecus aethiops
COS Cells
Cullin Proteins - genetics
Cullin Proteins - physiology
GROWTH
HEART
IN VITRO
IN VIVO
KIDNEYS
MAP Kinase Signaling System - physiology
MAPK
Molecular Sequence Data
Mutation
NUCLEOTIDES
Phosphorylation
RABBITS
Receptors, Vasopressin - genetics
Receptors, Vasopressin - physiology
Signal Transduction
Transfection
Truncated VACM-1/cul-5
VACM-1/cul-5
VASOPRESSIN
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Summary:We have sequenced a 4.9 kb clone (KLB22) which shares 99% sequence homology with the rabbit vasopressin-activated calcium mobilizing (VACM-1) protein. The 5′ terminus sequence of KLB22 cDNA (nucleotides 1–1961) is continuous and overlapping with nucleotides 1226–3186 of the VACM-1 cDNA sequence. The 3′UTR of KLB22 cDNA extends beyond the 3′UTR of VACM-1 by 2999 nt. KLB22 cDNA encodes a 497 amino acid protein, which putatively begins at Met 284 of the 780 amino acid VACM-1 protein. The in vitro translation of KLB22 cDNA yields a 59 kDa protein. When expressed in cos-1 cells, the truncated VACM-1 protein localizes to the nucleus. KLB22 cDNA transfected cells show increased growth rates and increased levels of phosphorylated MAPK when compared to the vector or to VACM-1 cDNA transfected cells. Finally, in vivo, KLB22 protein expression is tissue specific and can be detected in kidney and in heart atrium. These results suggest that truncated VACM-1 cDNA (KLB22) increases cell proliferation through a MAPK pathway.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.02.197