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Truncated form of VACM-1/cul-5 with an extended 3′ untranslated region stimulates cell growth via a MAPK-dependent pathway
We have sequenced a 4.9 kb clone (KLB22) which shares 99% sequence homology with the rabbit vasopressin-activated calcium mobilizing (VACM-1) protein. The 5′ terminus sequence of KLB22 cDNA (nucleotides 1–1961) is continuous and overlapping with nucleotides 1226–3186 of the VACM-1 cDNA sequence. The...
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| Published in: | Biochemical and biophysical research communications 2006-05, Vol.343 (4), p.1086-1093 |
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| cites | cdi_FETCH-LOGICAL-c413t-8dc366a38eea690f7d828bd84c8023eeb4915ac1ed0d54d6164589b91081333d3 |
| container_end_page | 1093 |
| container_issue | 4 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 343 |
| creator | Sartor, Ashleigh Kossoris, J.B. Wilcox, R. Shearer, R. Zeneberg, A.E. Zhao, P. Lazdins, I. Burnatowska-Hledin, Maria A. |
| description | We have sequenced a 4.9
kb clone (KLB22) which shares 99% sequence homology with the rabbit vasopressin-activated calcium mobilizing (VACM-1) protein. The 5′ terminus sequence of KLB22 cDNA (nucleotides 1–1961) is continuous and overlapping with nucleotides 1226–3186 of the VACM-1 cDNA sequence. The 3′UTR of KLB22 cDNA extends beyond the 3′UTR of VACM-1 by 2999
nt. KLB22 cDNA encodes a 497 amino acid protein, which putatively begins at Met 284 of the 780 amino acid VACM-1 protein. The in vitro translation of KLB22 cDNA yields a 59
kDa protein. When expressed in cos-1 cells, the truncated VACM-1 protein localizes to the nucleus. KLB22 cDNA transfected cells show increased growth rates and increased levels of phosphorylated MAPK when compared to the vector or to VACM-1 cDNA transfected cells. Finally, in vivo, KLB22 protein expression is tissue specific and can be detected in kidney and in heart atrium. These results suggest that truncated VACM-1 cDNA (KLB22) increases cell proliferation through a MAPK pathway. |
| doi_str_mv | 10.1016/j.bbrc.2006.02.197 |
| format | article |
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kb clone (KLB22) which shares 99% sequence homology with the rabbit vasopressin-activated calcium mobilizing (VACM-1) protein. The 5′ terminus sequence of KLB22 cDNA (nucleotides 1–1961) is continuous and overlapping with nucleotides 1226–3186 of the VACM-1 cDNA sequence. The 3′UTR of KLB22 cDNA extends beyond the 3′UTR of VACM-1 by 2999
nt. KLB22 cDNA encodes a 497 amino acid protein, which putatively begins at Met 284 of the 780 amino acid VACM-1 protein. The in vitro translation of KLB22 cDNA yields a 59
kDa protein. When expressed in cos-1 cells, the truncated VACM-1 protein localizes to the nucleus. KLB22 cDNA transfected cells show increased growth rates and increased levels of phosphorylated MAPK when compared to the vector or to VACM-1 cDNA transfected cells. Finally, in vivo, KLB22 protein expression is tissue specific and can be detected in kidney and in heart atrium. These results suggest that truncated VACM-1 cDNA (KLB22) increases cell proliferation through a MAPK pathway.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2006.02.197</identifier><identifier>PMID: 16581022</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>3' Untranslated Regions ; 60 APPLIED LIFE SCIENCES ; Amino Acid Sequence ; AMINO ACIDS ; Animals ; CALCIUM ; Cell growth ; Cell Nucleus - metabolism ; CELL PROLIFERATION ; Cercopithecus aethiops ; COS Cells ; Cullin Proteins - genetics ; Cullin Proteins - physiology ; GROWTH ; HEART ; IN VITRO ; IN VIVO ; KIDNEYS ; MAP Kinase Signaling System - physiology ; MAPK ; Molecular Sequence Data ; Mutation ; NUCLEOTIDES ; Phosphorylation ; RABBITS ; Receptors, Vasopressin - genetics ; Receptors, Vasopressin - physiology ; Signal Transduction ; Transfection ; Truncated VACM-1/cul-5 ; VACM-1/cul-5 ; VASOPRESSIN</subject><ispartof>Biochemical and biophysical research communications, 2006-05, Vol.343 (4), p.1086-1093</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-8dc366a38eea690f7d828bd84c8023eeb4915ac1ed0d54d6164589b91081333d3</citedby><cites>FETCH-LOGICAL-c413t-8dc366a38eea690f7d828bd84c8023eeb4915ac1ed0d54d6164589b91081333d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16581022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/20798952$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Sartor, Ashleigh</creatorcontrib><creatorcontrib>Kossoris, J.B.</creatorcontrib><creatorcontrib>Wilcox, R.</creatorcontrib><creatorcontrib>Shearer, R.</creatorcontrib><creatorcontrib>Zeneberg, A.E.</creatorcontrib><creatorcontrib>Zhao, P.</creatorcontrib><creatorcontrib>Lazdins, I.</creatorcontrib><creatorcontrib>Burnatowska-Hledin, Maria A.</creatorcontrib><title>Truncated form of VACM-1/cul-5 with an extended 3′ untranslated region stimulates cell growth via a MAPK-dependent pathway</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We have sequenced a 4.9
kb clone (KLB22) which shares 99% sequence homology with the rabbit vasopressin-activated calcium mobilizing (VACM-1) protein. The 5′ terminus sequence of KLB22 cDNA (nucleotides 1–1961) is continuous and overlapping with nucleotides 1226–3186 of the VACM-1 cDNA sequence. The 3′UTR of KLB22 cDNA extends beyond the 3′UTR of VACM-1 by 2999
nt. KLB22 cDNA encodes a 497 amino acid protein, which putatively begins at Met 284 of the 780 amino acid VACM-1 protein. The in vitro translation of KLB22 cDNA yields a 59
kDa protein. When expressed in cos-1 cells, the truncated VACM-1 protein localizes to the nucleus. KLB22 cDNA transfected cells show increased growth rates and increased levels of phosphorylated MAPK when compared to the vector or to VACM-1 cDNA transfected cells. Finally, in vivo, KLB22 protein expression is tissue specific and can be detected in kidney and in heart atrium. These results suggest that truncated VACM-1 cDNA (KLB22) increases cell proliferation through a MAPK pathway.</description><subject>3' Untranslated Regions</subject><subject>60 APPLIED LIFE SCIENCES</subject><subject>Amino Acid Sequence</subject><subject>AMINO ACIDS</subject><subject>Animals</subject><subject>CALCIUM</subject><subject>Cell growth</subject><subject>Cell Nucleus - metabolism</subject><subject>CELL PROLIFERATION</subject><subject>Cercopithecus aethiops</subject><subject>COS Cells</subject><subject>Cullin Proteins - genetics</subject><subject>Cullin Proteins - physiology</subject><subject>GROWTH</subject><subject>HEART</subject><subject>IN VITRO</subject><subject>IN VIVO</subject><subject>KIDNEYS</subject><subject>MAP Kinase Signaling System - physiology</subject><subject>MAPK</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>NUCLEOTIDES</subject><subject>Phosphorylation</subject><subject>RABBITS</subject><subject>Receptors, Vasopressin - genetics</subject><subject>Receptors, Vasopressin - physiology</subject><subject>Signal Transduction</subject><subject>Transfection</subject><subject>Truncated VACM-1/cul-5</subject><subject>VACM-1/cul-5</subject><subject>VASOPRESSIN</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkc2O0zAUhSMEYsrAC7BAlpDYJXOvk7ixxKaq-BMzgsWA2FmOfTNNlTrFdqaMxIJn4pF4EhxaiR2sLF1_5-iee7LsKUKBgOJiW7StNwUHEAXwAuXyXrZAkJBzhOp-toD0k3OJX86yRyFsARArIR9mZyjqBoHzRfb92k_O6EiWdaPfsbFjn1frqxwvzDTkNTv0ccO0Y_QtkrOJKn_9-MkmF712Yfij83TTj46F2O-meRKYoWFgN348JO1tr5lmV6uP73NL-9nDRbbXcXPQd4-zB50eAj05vefZp9evrtdv88sPb96tV5e5qbCMeWNNKYQuGyItJHRL2_CmtU1lGuAlUVtJrLVBsmDrygoUVd3IViI0WJalLc-z50ffMS2pgukjmY0ZnSMTFYelbGTNE_XiSO39-HWiENWuD3MU7WicghJLCXVZV_8FcYnJUkAC-RE0fgzBU6f2vt9pf6cQ1Fyh2qq5QjVXqICrVGESPTu5T-2O7F_JqbMEvDwClE5225OfE5EzZHs_B7Jj_y__39_QrL8</recordid><startdate>20060519</startdate><enddate>20060519</enddate><creator>Sartor, Ashleigh</creator><creator>Kossoris, J.B.</creator><creator>Wilcox, R.</creator><creator>Shearer, R.</creator><creator>Zeneberg, A.E.</creator><creator>Zhao, P.</creator><creator>Lazdins, I.</creator><creator>Burnatowska-Hledin, Maria A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20060519</creationdate><title>Truncated form of VACM-1/cul-5 with an extended 3′ untranslated region stimulates cell growth via a MAPK-dependent pathway</title><author>Sartor, Ashleigh ; Kossoris, J.B. ; Wilcox, R. ; Shearer, R. ; Zeneberg, A.E. ; Zhao, P. ; Lazdins, I. ; Burnatowska-Hledin, Maria A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-8dc366a38eea690f7d828bd84c8023eeb4915ac1ed0d54d6164589b91081333d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>3' Untranslated Regions</topic><topic>60 APPLIED LIFE SCIENCES</topic><topic>Amino Acid Sequence</topic><topic>AMINO ACIDS</topic><topic>Animals</topic><topic>CALCIUM</topic><topic>Cell growth</topic><topic>Cell Nucleus - metabolism</topic><topic>CELL PROLIFERATION</topic><topic>Cercopithecus aethiops</topic><topic>COS Cells</topic><topic>Cullin Proteins - genetics</topic><topic>Cullin Proteins - physiology</topic><topic>GROWTH</topic><topic>HEART</topic><topic>IN VITRO</topic><topic>IN VIVO</topic><topic>KIDNEYS</topic><topic>MAP Kinase Signaling System - physiology</topic><topic>MAPK</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>NUCLEOTIDES</topic><topic>Phosphorylation</topic><topic>RABBITS</topic><topic>Receptors, Vasopressin - genetics</topic><topic>Receptors, Vasopressin - physiology</topic><topic>Signal Transduction</topic><topic>Transfection</topic><topic>Truncated VACM-1/cul-5</topic><topic>VACM-1/cul-5</topic><topic>VASOPRESSIN</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sartor, Ashleigh</creatorcontrib><creatorcontrib>Kossoris, J.B.</creatorcontrib><creatorcontrib>Wilcox, R.</creatorcontrib><creatorcontrib>Shearer, R.</creatorcontrib><creatorcontrib>Zeneberg, A.E.</creatorcontrib><creatorcontrib>Zhao, P.</creatorcontrib><creatorcontrib>Lazdins, I.</creatorcontrib><creatorcontrib>Burnatowska-Hledin, Maria A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sartor, Ashleigh</au><au>Kossoris, J.B.</au><au>Wilcox, R.</au><au>Shearer, R.</au><au>Zeneberg, A.E.</au><au>Zhao, P.</au><au>Lazdins, I.</au><au>Burnatowska-Hledin, Maria A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Truncated form of VACM-1/cul-5 with an extended 3′ untranslated region stimulates cell growth via a MAPK-dependent pathway</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-05-19</date><risdate>2006</risdate><volume>343</volume><issue>4</issue><spage>1086</spage><epage>1093</epage><pages>1086-1093</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We have sequenced a 4.9
kb clone (KLB22) which shares 99% sequence homology with the rabbit vasopressin-activated calcium mobilizing (VACM-1) protein. The 5′ terminus sequence of KLB22 cDNA (nucleotides 1–1961) is continuous and overlapping with nucleotides 1226–3186 of the VACM-1 cDNA sequence. The 3′UTR of KLB22 cDNA extends beyond the 3′UTR of VACM-1 by 2999
nt. KLB22 cDNA encodes a 497 amino acid protein, which putatively begins at Met 284 of the 780 amino acid VACM-1 protein. The in vitro translation of KLB22 cDNA yields a 59
kDa protein. When expressed in cos-1 cells, the truncated VACM-1 protein localizes to the nucleus. KLB22 cDNA transfected cells show increased growth rates and increased levels of phosphorylated MAPK when compared to the vector or to VACM-1 cDNA transfected cells. Finally, in vivo, KLB22 protein expression is tissue specific and can be detected in kidney and in heart atrium. These results suggest that truncated VACM-1 cDNA (KLB22) increases cell proliferation through a MAPK pathway.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16581022</pmid><doi>10.1016/j.bbrc.2006.02.197</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2006-05, Vol.343 (4), p.1086-1093 |
| issn | 0006-291X 1090-2104 |
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| recordid | cdi_osti_scitechconnect_20798952 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | 3' Untranslated Regions 60 APPLIED LIFE SCIENCES Amino Acid Sequence AMINO ACIDS Animals CALCIUM Cell growth Cell Nucleus - metabolism CELL PROLIFERATION Cercopithecus aethiops COS Cells Cullin Proteins - genetics Cullin Proteins - physiology GROWTH HEART IN VITRO IN VIVO KIDNEYS MAP Kinase Signaling System - physiology MAPK Molecular Sequence Data Mutation NUCLEOTIDES Phosphorylation RABBITS Receptors, Vasopressin - genetics Receptors, Vasopressin - physiology Signal Transduction Transfection Truncated VACM-1/cul-5 VACM-1/cul-5 VASOPRESSIN |
| title | Truncated form of VACM-1/cul-5 with an extended 3′ untranslated region stimulates cell growth via a MAPK-dependent pathway |
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