The human RNA polymerase II interacts with the terminal stem–loop regions of the hepatitis delta virus RNA genome

Abstract The hepatitis delta virus (HDV) is an RNA virus that depends on DNA-dependent RNA polymerase (RNAP) for its transcription and replication. While it is generally accepted that RNAP II is involved in HDV replication, its interaction with HDV RNA requires confirmation. A monoclonal antibody sp...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2007-01, Vol.357 (1), p.68-78
Main Authors: Greco-Stewart, Valerie S, Miron, Paul, Abrahem, Abrahem, Pelchat, Martin
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Base Sequence
Co-immunoprecipitation
DNA
HELA CELLS
Hepatitis D virus
Hepatitis delta virus
Hepatitis Delta Virus - physiology
Humans
Infectious Disease
INFECTIOUS HEPATITIS
Molecular Sequence Data
MONOCLONAL ANTIBODIES
Nucleic Acid Conformation
PROMOTERS
Protein Binding
RNA
RNA polymerase II
RNA Polymerase II - metabolism
RNA promoter
RNA virus
RNA, Viral - chemistry
RNA, Viral - genetics
RNA, Viral - metabolism
TRANSCRIPTION
Transcription Initiation Site - physiology
Virus Replication
VIRUSES
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Summary:Abstract The hepatitis delta virus (HDV) is an RNA virus that depends on DNA-dependent RNA polymerase (RNAP) for its transcription and replication. While it is generally accepted that RNAP II is involved in HDV replication, its interaction with HDV RNA requires confirmation. A monoclonal antibody specific to the carboxy terminal domain of the largest subunit of RNAP II was used to establish the association of RNAP II with both polarities of HDV RNA in HeLa cells. Co-immunoprecipitations using HeLa nuclear extract revealed that RNAP II interacts with HDV-derived RNAs at sites located within the terminal stem–loop domains of both polarities of HDV RNA. Analysis of these regions revealed a strong selection to maintain a rod-like conformation and demonstrated several conserved features. These results provide the first direct evidence of an association between human RNAP II and HDV RNA and suggest two transcription start sites on both polarities of HDV RNA.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2006.08.010