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ETV4 and Myeov knockdown impairs colon cancer cell line proliferation and invasion

We have identified novel colorectal cancer-associated genes using NCBI’s UNIGENE cDNA libraries. Colon cancer libraries were examined using Digital Differential Display and disease-associated genes were selected. Among these were ETV4 and MYEOV, novel colorectal cancer-associated genes. Samples of m...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-06, Vol.345 (1), p.216-221
Main Authors: Moss, Alan C., Lawlor, Garrett, Murray, David, Tighe, Dónal, Madden, Stephen F., Mulligan, Anne-Marie, Keane, Conor O., Brady, Hugh R., Doran, Peter P., MacMathuna, Padraic
Format: Article
Language:English
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Summary:We have identified novel colorectal cancer-associated genes using NCBI’s UNIGENE cDNA libraries. Colon cancer libraries were examined using Digital Differential Display and disease-associated genes were selected. Among these were ETV4 and MYEOV, novel colorectal cancer-associated genes. Samples of matched normal and neoplastic colon were obtained from human subjects and gene expression was quantified using real-time PCR. ETV4 gene expression was significantly increased in colonic neoplasia in comparison to matched normal colonic tissue ( p < 0.05). Myeov expression was also increased in colon neoplasia in comparison to matched normal tissue. The effect of siRNA-mediated knockdown of ETV4 and Myeov on cell proliferation and invasion was assessed. ETV4 knockdown resulted in a 90% decrease in cell proliferation ( p < 0.05) and a 67% decrease in cell invasion. Myeov knockdown resulted in a 48% decrease in cell proliferation ( p < 0.05) and a 36% decrease in cell invasion. These data suggest that ETV4 and Myeov may provide novel targets for therapeutic intervention.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.04.094