A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells

Traditionally, undifferentiated human embryonic stem cells (hESCs) are maintained on mouse embryonic fibroblast (MEF) cells or on matrigel with an MEF-conditioned medium (CM), which hampers the clinical applications of hESCs due to the contamination by animal pathogens. Here we report a novel chemic...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-07, Vol.346 (1), p.131-139
Main Authors: Liu, Yanxia, Song, Zhihua, Zhao, Yang, Qin, Han, Cai, Jun, Zhang, Hong, Yu, Tianxin, Jiang, Siming, Wang, Guangwen, Ding, Mingxiao, Deng, Hongkui
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
APOPTOSIS
bFGF
Cell Culture Techniques - methods
Cell Differentiation
CELL PROLIFERATION
Chemical-defined medium
CULTURE MEDIA
Culture Media - chemistry
Fibroblast Growth Factor 2
FIBROBLASTS
Fibronectin
GROWTH
GROWTH FACTORS
HLA-B27 Antigen
Human embryonic stem cells
Humans
IN VITRO
KARYOTYPE
Long-term culture
MICE
N2B27
PATHOGENS
Pluripotency
Pluripotent Stem Cells - cytology
Self-renewal
STEM CELLS
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Traditionally, undifferentiated human embryonic stem cells (hESCs) are maintained on mouse embryonic fibroblast (MEF) cells or on matrigel with an MEF-conditioned medium (CM), which hampers the clinical applications of hESCs due to the contamination by animal pathogens. Here we report a novel chemical-defined medium using DMEM/F12 supplemented with N2, B27, and basic fibroblast growth factor (bFGF) [termed NBF]. This medium can support prolonged self-renewal of hESCs. hESCs cultured in NBF maintain an undifferentiated state and normal karyotype, are able to form embryoid bodies in vitro, and differentiate into three germ layers and extraembryonic cells. Furthermore, we find that hESCs cultured in NBF possess a low apoptosis rate and a high proliferation rate compared with those cultured in MEF-CM. Our findings provide a novel, simplified chemical-defined culture medium suitable for further therapeutic applications and developmental studies of hESCs.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.05.086