Arginine-rich intracellular delivery peptides noncovalently transport protein into living cells

Plasma membranes of plant or animal cells are generally impermeable to peptides or proteins. Many basic peptides have previously been investigated and covalently cross-linked with cargoes for cellular internalization. In the current study, we demonstrate that arginine-rich intracellular delivery (AI...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-08, Vol.346 (3), p.758-767
Main Authors: Wang, Ya-Hui, Chen, Chung-Pin, Chan, Ming-Huan, Chang, Microsugar, Hou, Yu-Wun, Chen, Hwei-Hsien, Hsu, Hui-Ru, Liu, Kevin, Lee, Han-Jung
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
Animals
ARGININE
Arginine - metabolism
Cell Line
Cell Line, Tumor
Cell penetrating peptide
Cell Survival
Cells - cytology
Cells - metabolism
Cellular internalization
Cricetinae
GALACTOSIDASE
Green fluorescent protein
Humans
IN VIVO
Liver - cytology
Liver - metabolism
Mice
Onions - cytology
Onions - metabolism
PEPTIDES
Peptides - chemistry
Peptides - genetics
Peptides - metabolism
Peptides - toxicity
PLANT CELLS
Plasmids - genetics
Protein transduction domain
Protein Transport
Skin - cytology
Skin - metabolism
Transdermal delivery
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Summary:Plasma membranes of plant or animal cells are generally impermeable to peptides or proteins. Many basic peptides have previously been investigated and covalently cross-linked with cargoes for cellular internalization. In the current study, we demonstrate that arginine-rich intracellular delivery (AID) peptides are able to deliver fluorescent proteins or β-galactosidase enzyme into animal and plant cells, as well as animal tissue. Cellular internalization and transdermal delivery of protein could be mediated by effective and nontoxic AID peptides in a neither fusion protein nor conjugation fashion. Therefore, noncovalent AID peptides may provide a useful strategy to have active proteins function in living cells and tissues in vivo.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.05.205