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Arginine-rich intracellular delivery peptides noncovalently transport protein into living cells
Plasma membranes of plant or animal cells are generally impermeable to peptides or proteins. Many basic peptides have previously been investigated and covalently cross-linked with cargoes for cellular internalization. In the current study, we demonstrate that arginine-rich intracellular delivery (AI...
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| Published in: | Biochemical and biophysical research communications 2006-08, Vol.346 (3), p.758-767 |
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| Main Authors: | , , , , , , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c413t-3cc038d7b5fa2ef29a86cacb1ac197fcd30ee02d200a342ac6a1ba6a30da2ffc3 |
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| cites | cdi_FETCH-LOGICAL-c413t-3cc038d7b5fa2ef29a86cacb1ac197fcd30ee02d200a342ac6a1ba6a30da2ffc3 |
| container_end_page | 767 |
| container_issue | 3 |
| container_start_page | 758 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 346 |
| creator | Wang, Ya-Hui Chen, Chung-Pin Chan, Ming-Huan Chang, Microsugar Hou, Yu-Wun Chen, Hwei-Hsien Hsu, Hui-Ru Liu, Kevin Lee, Han-Jung |
| description | Plasma membranes of plant or animal cells are generally impermeable to peptides or proteins. Many basic peptides have previously been investigated and covalently cross-linked with cargoes for cellular internalization. In the current study, we demonstrate that arginine-rich intracellular delivery (AID) peptides are able to deliver fluorescent proteins or β-galactosidase enzyme into animal and plant cells, as well as animal tissue. Cellular internalization and transdermal delivery of protein could be mediated by effective and nontoxic AID peptides in a neither fusion protein nor conjugation fashion. Therefore, noncovalent AID peptides may provide a useful strategy to have active proteins function in living cells and tissues
in vivo. |
| doi_str_mv | 10.1016/j.bbrc.2006.05.205 |
| format | article |
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in vivo.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>ANIMAL CELLS</subject><subject>ANIMAL TISSUES</subject><subject>Animals</subject><subject>ARGININE</subject><subject>Arginine - metabolism</subject><subject>Cell Line</subject><subject>Cell Line, Tumor</subject><subject>Cell penetrating peptide</subject><subject>Cell Survival</subject><subject>Cells - cytology</subject><subject>Cells - metabolism</subject><subject>Cellular internalization</subject><subject>Cricetinae</subject><subject>GALACTOSIDASE</subject><subject>Green fluorescent protein</subject><subject>Humans</subject><subject>IN VIVO</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Mice</subject><subject>Onions - cytology</subject><subject>Onions - metabolism</subject><subject>PEPTIDES</subject><subject>Peptides - chemistry</subject><subject>Peptides - genetics</subject><subject>Peptides - metabolism</subject><subject>Peptides - toxicity</subject><subject>PLANT CELLS</subject><subject>Plasmids - genetics</subject><subject>Protein transduction domain</subject><subject>Protein Transport</subject><subject>Skin - cytology</subject><subject>Skin - metabolism</subject><subject>Transdermal delivery</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kEFr3DAQhUVpaTZp_0APwVDozZuRZGtt6CUsbVoI5NJCb0IejRMtXsmRtAv77yuzC7319A7zvcebx9gnDmsOXN3t1sMQcS0A1Braou0btuLQQy04NG_ZCsqlFj3_c8WuU9oBcN6o_j274mrTcaXUiun7-Oy881RHhy-V8zkapGk6TCZWliZ3pHiqZpqzs5QqHzyGo5nI5-lUFdanOcRczTFkcn7xh6qYnH-ulpj0gb0bzZTo40Vv2O_v335tf9SPTw8_t_ePNTZc5loiguzsZmhHI2gUvekUGhy4Qd5vRrQSiEDY8quRjTCoDB-MMhKsEeOI8oZ9PueGlJ1O6DLhCwbvCbMW0LWN7LpCfTlTpe_rgVLWe5eWnsZTOCTN-0ZxJWQBxRnEGFKKNOo5ur2JJ81BL-PrnV7G18v4GtqibTHdXtIPw57sP8tl7QJ8PQNUljg6iktR8kjWxaWnDe5_-X8BA7yYwg</recordid><startdate>20060804</startdate><enddate>20060804</enddate><creator>Wang, Ya-Hui</creator><creator>Chen, Chung-Pin</creator><creator>Chan, Ming-Huan</creator><creator>Chang, Microsugar</creator><creator>Hou, Yu-Wun</creator><creator>Chen, Hwei-Hsien</creator><creator>Hsu, Hui-Ru</creator><creator>Liu, Kevin</creator><creator>Lee, Han-Jung</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>OTOTI</scope></search><sort><creationdate>20060804</creationdate><title>Arginine-rich intracellular delivery peptides noncovalently transport protein into living cells</title><author>Wang, Ya-Hui ; 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| ispartof | Biochemical and biophysical research communications, 2006-08, Vol.346 (3), p.758-767 |
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| language | eng |
| recordid | cdi_osti_scitechconnect_20854388 |
| source | Elsevier |
| subjects | 60 APPLIED LIFE SCIENCES ANIMAL CELLS ANIMAL TISSUES Animals ARGININE Arginine - metabolism Cell Line Cell Line, Tumor Cell penetrating peptide Cell Survival Cells - cytology Cells - metabolism Cellular internalization Cricetinae GALACTOSIDASE Green fluorescent protein Humans IN VIVO Liver - cytology Liver - metabolism Mice Onions - cytology Onions - metabolism PEPTIDES Peptides - chemistry Peptides - genetics Peptides - metabolism Peptides - toxicity PLANT CELLS Plasmids - genetics Protein transduction domain Protein Transport Skin - cytology Skin - metabolism Transdermal delivery |
| title | Arginine-rich intracellular delivery peptides noncovalently transport protein into living cells |
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