A cytoskeleton-associated protein, TMAP/CKAP2, is involved in the proliferation of human foreskin fibroblasts

Previously, we reported the cloning of a cytoskeleton-associated protein, TMAP/CKAP2, which was up-regulated in primary human gastric cancers. Although TMAP/CKAP2 has been found to be expressed in most cancer cell lines examined, the function of CKAP2 is not known. In this study, we found that TMAP/...

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Published in:Biochemical and biophysical research communications 2006-09, Vol.348 (1), p.222-228
Main Authors: Jeon, Sang-Min, Choi, Bongkun, Hong, Kyung Uk, Kim, Eunhee, Seong, Yeon-Sun, Bae, Chang-Dae, Park, Joobae
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
CELL CYCLE
CELL PROLIFERATION
CKAP2
CLONING
Cytoskeletal Proteins - metabolism
Cytoskeleton
FIBROBLASTS
Fibroblasts - cytology
Fibroblasts - metabolism
G1 Phase - physiology
Gene Expression Regulation
Humans
Microtubule-Associated Proteins - metabolism
MICROTUBULES
NEOPLASMS
PHOSPHORYLATION
Proliferation
PROTEINS
TMAP
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Summary:Previously, we reported the cloning of a cytoskeleton-associated protein, TMAP/CKAP2, which was up-regulated in primary human gastric cancers. Although TMAP/CKAP2 has been found to be expressed in most cancer cell lines examined, the function of CKAP2 is not known. In this study, we found that TMAP/CKAP2 was not expressed in G0/G1 arrested HFFs, but that it was expressed in actively dividing cells. After initiating the cell cycle, TMAP/CKAP2 levels remained low throughout most of the G1 phase, but gradually increased between late G1 and G2/M. Knockdown of TMAP/CKAP2 reduced pRB phosphorylation and increased p27 expression, and consequently reduced HFF proliferation, whereas constitutive TMAP/CKAP2 expression increased pRB phosphorylation and enhanced proliferation. Our results show that this novel cytoskeleton-associated protein is expressed cell cycle dependently and that it is involved in cell proliferation.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.07.046