Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts

Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the pos...

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Published in:Biochemical and biophysical research communications 2006-10, Vol.349 (3), p.995-1002
Main Authors: Ryoo, Zae Young, Jung, Boo Kyoung, Lee, Sang Ryeul, Kim, Myoung Ok, Kim, Sung Hyun, Kim, Hyo Jin, Ahn, Jung Yong, Lee, Tae-Hoon, Cho, Youl Hee, Park, Jae Hak, Kim, Jin Kyeoung
Format: Article
Language:English
Subjects:
3T3 Cells
60 APPLIED LIFE SCIENCES
AGAR
Animals
CELL CYCLE
CELL PROLIFERATION
Cell Transformation, Neoplastic - genetics
Cell Transformation, Neoplastic - metabolism
Cell Transformation, Neoplastic - pathology
Culture Media
Female
Fibroblast
FIBROBLASTS
Gene Expression Regulation, Neoplastic
GENES
Genetic Vectors - genetics
Immunohistochemistry
IMUP-1
IMUP-2
IN VIVO
MICE
Mice, Nude
NEOPLASMS
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
PHENOTYPE
PROTEINS
Serum
SV40
Transcription Factors - genetics
Transcription Factors - metabolism
Tumorigenicity
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Summary:Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties, anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play an important role in acquiring a transformed phenotype, tumorigenicity in vivo, and be related to cellular proliferation.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.08.137