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Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts
Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the pos...
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| Published in: | Biochemical and biophysical research communications 2006-10, Vol.349 (3), p.995-1002 |
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| cites | cdi_FETCH-LOGICAL-c413t-e4df8aeba979dbb9e0f608278bec80025dd32d666086d75e532b751919ff6ed03 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 349 |
| creator | Ryoo, Zae Young Jung, Boo Kyoung Lee, Sang Ryeul Kim, Myoung Ok Kim, Sung Hyun Kim, Hyo Jin Ahn, Jung Yong Lee, Tae-Hoon Cho, Youl Hee Park, Jae Hak Kim, Jin Kyeoung |
| description | Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties, anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play an important role in acquiring a transformed phenotype, tumorigenicity
in vivo, and be related to cellular proliferation. |
| doi_str_mv | 10.1016/j.bbrc.2006.08.137 |
| format | article |
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in vivo, and be related to cellular proliferation.</description><subject>3T3 Cells</subject><subject>60 APPLIED LIFE SCIENCES</subject><subject>AGAR</subject><subject>Animals</subject><subject>CELL CYCLE</subject><subject>CELL PROLIFERATION</subject><subject>Cell Transformation, Neoplastic - genetics</subject><subject>Cell Transformation, Neoplastic - metabolism</subject><subject>Cell Transformation, Neoplastic - pathology</subject><subject>Culture Media</subject><subject>Female</subject><subject>Fibroblast</subject><subject>FIBROBLASTS</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>GENES</subject><subject>Genetic Vectors - genetics</subject><subject>Immunohistochemistry</subject><subject>IMUP-1</subject><subject>IMUP-2</subject><subject>IN VIVO</subject><subject>MICE</subject><subject>Mice, Nude</subject><subject>NEOPLASMS</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>PHENOTYPE</subject><subject>PROTEINS</subject><subject>Serum</subject><subject>SV40</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Tumorigenicity</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkc1u1DAUhS0EosPAC7BAlpC6y9R2EseW2KAKaKWqbIrEzvLPDfUoiYPttPQZeGmcmZHYwcqWfb7je3wQekvJjhLKL_Y7Y6LdMUL4jogdrbtnaEOJJBWjpHmONqTcVEzS72foVUp7QihtuHyJziiXnLWcbdDvWwjzoFP2Fueop9SHOOrsw4T15HBexhD9D5gg-YR1SsF6ncHhR5_vcXiACL_mCCmtQOixH5e5ogf0sGX4wGI_YbsMeYkFvb2-uqjvajyGJQHuvYnBrBOk1-hFr4cEb07rFn37_Onu8qq6-frl-vLjTWUbWucKGtcLDUbLTjpjJJCeE8E6YcAKQljrXM0c5-WQu66Ftmama6mksu85OFJv0fujbyixVbI-g723YZrAZsWIaJu2PLRF50fVHMPPBVJWo08WhkFPUCZXXIhO1kz-V0hlLUQteBGyo9DGkFKEXs3Rjzo-KUrU2qjaq7VRtTaqiFCl0QK9O7kvZgT3FzlVWAQfjgIoX_bgIa6JYLLgfFwDueD_5f8HfSaz2g</recordid><startdate>20061027</startdate><enddate>20061027</enddate><creator>Ryoo, Zae Young</creator><creator>Jung, Boo Kyoung</creator><creator>Lee, Sang Ryeul</creator><creator>Kim, Myoung Ok</creator><creator>Kim, Sung Hyun</creator><creator>Kim, Hyo Jin</creator><creator>Ahn, Jung Yong</creator><creator>Lee, Tae-Hoon</creator><creator>Cho, Youl Hee</creator><creator>Park, Jae Hak</creator><creator>Kim, Jin Kyeoung</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20061027</creationdate><title>Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts</title><author>Ryoo, Zae Young ; Jung, Boo Kyoung ; Lee, Sang Ryeul ; Kim, Myoung Ok ; Kim, Sung Hyun ; Kim, Hyo Jin ; Ahn, Jung Yong ; Lee, Tae-Hoon ; Cho, Youl Hee ; Park, Jae Hak ; Kim, Jin Kyeoung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-e4df8aeba979dbb9e0f608278bec80025dd32d666086d75e532b751919ff6ed03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>3T3 Cells</topic><topic>60 APPLIED LIFE SCIENCES</topic><topic>AGAR</topic><topic>Animals</topic><topic>CELL CYCLE</topic><topic>CELL PROLIFERATION</topic><topic>Cell Transformation, Neoplastic - genetics</topic><topic>Cell Transformation, Neoplastic - metabolism</topic><topic>Cell Transformation, Neoplastic - pathology</topic><topic>Culture Media</topic><topic>Female</topic><topic>Fibroblast</topic><topic>FIBROBLASTS</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>GENES</topic><topic>Genetic Vectors - genetics</topic><topic>Immunohistochemistry</topic><topic>IMUP-1</topic><topic>IMUP-2</topic><topic>IN VIVO</topic><topic>MICE</topic><topic>Mice, Nude</topic><topic>NEOPLASMS</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>PHENOTYPE</topic><topic>PROTEINS</topic><topic>Serum</topic><topic>SV40</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Tumorigenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ryoo, Zae Young</creatorcontrib><creatorcontrib>Jung, Boo Kyoung</creatorcontrib><creatorcontrib>Lee, Sang Ryeul</creatorcontrib><creatorcontrib>Kim, Myoung Ok</creatorcontrib><creatorcontrib>Kim, Sung Hyun</creatorcontrib><creatorcontrib>Kim, Hyo Jin</creatorcontrib><creatorcontrib>Ahn, Jung Yong</creatorcontrib><creatorcontrib>Lee, Tae-Hoon</creatorcontrib><creatorcontrib>Cho, Youl Hee</creatorcontrib><creatorcontrib>Park, Jae Hak</creatorcontrib><creatorcontrib>Kim, Jin Kyeoung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ryoo, Zae Young</au><au>Jung, Boo Kyoung</au><au>Lee, Sang Ryeul</au><au>Kim, Myoung Ok</au><au>Kim, Sung Hyun</au><au>Kim, Hyo Jin</au><au>Ahn, Jung Yong</au><au>Lee, Tae-Hoon</au><au>Cho, Youl Hee</au><au>Park, Jae Hak</au><au>Kim, Jin Kyeoung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-10-27</date><risdate>2006</risdate><volume>349</volume><issue>3</issue><spage>995</spage><epage>1002</epage><pages>995-1002</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties, anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play an important role in acquiring a transformed phenotype, tumorigenicity
in vivo, and be related to cellular proliferation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16962562</pmid><doi>10.1016/j.bbrc.2006.08.137</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2006-10, Vol.349 (3), p.995-1002 |
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| source | ScienceDirect Journals |
| subjects | 3T3 Cells 60 APPLIED LIFE SCIENCES AGAR Animals CELL CYCLE CELL PROLIFERATION Cell Transformation, Neoplastic - genetics Cell Transformation, Neoplastic - metabolism Cell Transformation, Neoplastic - pathology Culture Media Female Fibroblast FIBROBLASTS Gene Expression Regulation, Neoplastic GENES Genetic Vectors - genetics Immunohistochemistry IMUP-1 IMUP-2 IN VIVO MICE Mice, Nude NEOPLASMS Nuclear Proteins - genetics Nuclear Proteins - metabolism PHENOTYPE PROTEINS Serum SV40 Transcription Factors - genetics Transcription Factors - metabolism Tumorigenicity |
| title | Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts |
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