Comparison between basal and apical dendritic spines in estrogen-induced rapid spinogenesis of CA1 principal neurons in the adult hippocampus

• Published in: Biochemical and biophysical research communications 2006-12, Vol.351 (2), p.553-558
• Main Authors: Murakami, Gen, Tsurugizawa, Tomokazu, Hatanaka, Yusuke, Komatsuzaki, Yoshimasa, Tanabe, Nobuaki, Mukai, Hideo, Hojo, Yasushi, Kominami, Shiro, Yamazaki, Takeshi, Kimoto, Tetsuya, Kawato, Suguru
• Format: Article
• Language: English
• Subjects: 6-Cyano-7-nitroquinoxaline-2,3-dione - pharmacology; 60 APPLIED LIFE SCIENCES; Animals; CA1; Dendritic Spines - drug effects; Dendritic Spines - physiology; ESTRADIOL; Estradiol - pharmacology; Estrogen; Estrogen receptor; Estrogen Receptor alpha - agonists; Estrogen Receptor alpha - antagonists & inhibitors; Estrogen Receptor alpha - metabolism; Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors; Extracellular Signal-Regulated MAP Kinases - metabolism; Flavonoids - pharmacology; HIPPOCAMPUS; Hippocampus - cytology; Hippocampus - drug effects; Hippocampus - physiology; In Vitro Techniques; Male; MORPHOLOGY; NERVE CELLS; Neuronal Plasticity; Neurons - cytology; Neurons - drug effects; Neurons - physiology; Nitriles - pharmacology; Phenols; Pyramidal Cells - cytology; Pyramidal Cells - drug effects; Pyramidal Cells - physiology; Pyrazoles - pharmacology; RATS; Rats, Wistar; RECEPTORS; Receptors, AMPA - agonists; Receptors, AMPA - metabolism; Receptors, N-Methyl-D-Aspartate - agonists; Receptors, N-Methyl-D-Aspartate - metabolism; Spine; Synaptic plasticity; VERTEBRAE
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2006.10.066

Modulation of hippocampal synaptic plasticity by estrogen has been attracting much attention. Here, we demonstrated the rapid effect of 17β-estradiol on the density and morphology of spines in the stratum oriens (s.o., basal side) and in the stratum lacunosum-moleculare (s.l.m., apical side) by imaging Lucifer Yellow-injected CA1 neurons in adult male rat hippocampal slices, because spines in s.o. and s.l.m. have been poorly understood as compared with spines in the stratum radiatum. The application of 1 nM estradiol-induced a rapid increase in the density of spines of pyramidal neurons within 2 h. This increase by estradiol was blocked by Erk MAP kinase inhibitor and estrogen receptor inhibitor in both regions. Effect of blockade by agonists of AMPA receptors and NMDA receptors was different between s.o. and s.l.m. In both regions, ERα agonist PPT induced the same enhancing effect of spinogenesis as that induced by estradiol.