Functionality of NGF-protected PC12 cells following exposure to 6-hydroxydopamine

6-Hydroxydopamine (6-OHDA) is often used in models of Parkinson’s disease since it can selectively target and kill dopaminergic cells of the substantia nigra. In this study, pre-treatment of PC12 cells with nerve growth factor (NGF) inhibited apoptosis and necrosis by 6-OHDA, including caspase activ...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-12, Vol.351 (4), p.890-895
Main Authors: Kavanagh, Edel T., Loughlin, John P., Herbert, Kate Reed, Dockery, Peter, Samali, Afshin, Doyle, Karen M., Gorman, Adrienne M.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Animals
APOPTOSIS
Apoptosis - drug effects
Biomarkers - analysis
CATECHOLAMINES
Catecholamines - analysis
Cell Differentiation
Cell Proliferation
DOPAMINE
GROWTH FACTORS
HYDROXYLASES
LACTATE DEHYDROGENASE
NECROSIS
NERVE CELLS
Nerve Growth Factor - pharmacology
NERVOUS SYSTEM DISEASES
Neurite outgrowth
Neurites - drug effects
Neurites - physiology
Neurons - cytology
Neurons - drug effects
Oxidopamine - antagonists & inhibitors
Oxidopamine - toxicity
Parkinson’s disease
PC12 Cells
Rats
Tubulin - analysis
TYROSINE
Tyrosine 3-Monooxygenase - analysis
Tyrosine hydroxylase
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Summary:6-Hydroxydopamine (6-OHDA) is often used in models of Parkinson’s disease since it can selectively target and kill dopaminergic cells of the substantia nigra. In this study, pre-treatment of PC12 cells with nerve growth factor (NGF) inhibited apoptosis and necrosis by 6-OHDA, including caspase activity and lactate dehydrogenase release. Notably, cells exposed to 6-OHDA in the presence of NGF were subsequently capable of proliferation (when replated without NGF), or neurite outgrowth (with continued presence of NGF). Following 7 days growth in the presence of NGF, expression of βIII tubulin and tyrosine hydroxylase and increased intracellular catecholamines was detectable in PC12 cells, features characteristic of functional dopaminergic neurons. NGF-pre-treated PC12 cells retained expression of βIII-tubulin and tyrosine hydroxylase, but not catecholamine content following 6-OHDA exposure. These data indicate that NGF-protected cells maintained some aspects of functionality and were subsequently capable of proliferation or differentiation.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.10.104