Angiogenin-induced protein kinase B/Akt activation is necessary for angiogenesis but is independent of nuclear translocation of angiogenin in HUVE cells

Angiogenin, a potent angiogenic factor, binds to endothelial cells and is endocytosed and rapidly translocated to and concentrated in the nucleolus where it binds to DNA. In this study, we report that angiogenin induces transient phosphorylation of protein kinase B/Akt in cultured human umbilical ve...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-01, Vol.352 (2), p.509-513
Main Authors: Kim, Hye-Mi, Kang, Dong-Ku, Kim, Hak Yong, Kang, Sang Sun, Chang, Soo-Ik
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Active Transport, Cell Nucleus - physiology
Akt
Angiogenin
CALCIUM
CATTLE
Cell Nucleus - metabolism
Cells, Cultured
DNA
Dose-Response Relationship, Drug
EMBRYOS
Endothelial Cells - drug effects
Endothelial Cells - metabolism
FETAL MEMBRANES
Humans
IN VITRO
IN VIVO
MAGNESIUM
Neovascularization, Physiologic - physiology
Nuclear translocation
Oncogene Protein v-akt - metabolism
PH VALUE
PHOSPHATES
PHOSPHORYLATION
PROTEINS
Proto-Oncogene Proteins c-akt - metabolism
Ribonuclease, Pancreatic - administration & dosage
Ribonuclease, Pancreatic - metabolism
TRANSLOCATION
Umbilical Cord - cytology
Umbilical Cord - drug effects
Umbilical Cord - metabolism
VEINS
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Summary:Angiogenin, a potent angiogenic factor, binds to endothelial cells and is endocytosed and rapidly translocated to and concentrated in the nucleolus where it binds to DNA. In this study, we report that angiogenin induces transient phosphorylation of protein kinase B/Akt in cultured human umbilical vein endothelial (HUVE) cells. LY294002 inhibits the angiogenin-induced protein kinase B/Akt activation and also angiogenin-induced cell migration in vitro as well as angiogenesis in chick embryo chorioallantoic membrane in vivo without affecting nuclear translocation of angiogenin in HUVE cells. These results suggest that cross-talk between angiogenin and protein kinase B/Akt signaling pathways is essential for angiogenin-induced angiogenesis in vitro and in vivo, and that angiogenin-induced PKB/Akt activation is independent of nuclear translocation of angiogenin in HUVE cells.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.11.047