Live imaging of spindle pole disorganization in docetaxel-treated multicolor cells

Treatment of cells with docetaxel at low concentrations induces aberrant bipolar spindles of which two centrosomes stay at only one pole, and also induces multipolar spindles. To gain insight into the relations between centrosome impairment and structural defects of the spindle, live-cell imaging wa...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-06, Vol.357 (3), p.655-660
Main Authors: Sakaushi, Shinji, Nishida, Kumi, Minamikawa, Harumi, Fukada, Takashi, Oka, Shigenori, Sugimoto, Kenji
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Antineoplastic Agents - pharmacology
Aurora Kinases
Cell Division
Cell Line, Tumor
CELL MEMBRANES
Centrosome
Centrosome - drug effects
Centrosome - physiology
CHROMATIN
Docetaxel
Dose-Response Relationship, Drug
Fluorescent protein
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Humans
IMAGE PROCESSING
Interphase
Live-cell imaging
Microscopy, Fluorescence - methods
MITOSIS
Paclitaxel
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
PROTEINS
Spindle Apparatus - drug effects
Spindle Apparatus - physiology
Spindle pole
Taxoids - pharmacology
Time Factors
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Summary:Treatment of cells with docetaxel at low concentrations induces aberrant bipolar spindles of which two centrosomes stay at only one pole, and also induces multipolar spindles. To gain insight into the relations between centrosome impairment and structural defects of the spindle, live-cell imaging was performed on a human MDA Auro/imp/H3 cell line in which centrosomes/mitotic spindles, nuclear membrane and chromatin were simultaneously visualized by fluorescent proteins. In the presence of docetaxel at IC 50 concentration, the centrosomes did not segregate, and multiple aster-like structures ectopically arose around the disappearing nuclear membrane. Those ectopic structures formed an acentrosomal pole opposing to the two-centrosomes-containing pole. In late metaphase, one pole often fragmented into multiple spindle poles, leading multipolar division. These results suggest that spindle pole fragility may be induced by centrosome impairment, and collapse of the pole may contribute to induction of aneuploid daughter cells.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.03.205