Wp specific methylation of highly proliferated LCLs

The epigenetic regulation of viral genes may be important for the life cycle of EBV. We determined the methylation status of three viral promoters (Wp, Cp, Qp) from EBV B-lymphoblastoid cell lines (LCLs) by pyrosequencing. Our pyrosequencing data showed that the CpG region of Wp was methylated, but...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-06, Vol.358 (2), p.513-520
Main Authors: Park, Jung-Hoon, Jeon, Jae-Pil, Shim, Sung-Mi, Nam, Hye-Young, Kim, Joon-Woo, Han, Bok-Ghee, Lee, Suman
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
B-Lymphocytes - virology
Base Sequence
CELL PROLIFERATION
Cells, Cultured
CpG Islands - genetics
DNA Methylation
DNA, Viral - genetics
EBNA2
EBV
Epstein-Barr virus
Gene Expression Regulation, Viral - physiology
GENE REGULATION
GENES
Herpesvirus 4, Human - genetics
Humans
LIFE CYCLE
LMP1
LYMPHOMAS
METHYLATION
Molecular Sequence Data
Promoter Regions, Genetic - genetics
PROMOTERS
Viral Proteins - genetics
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Summary:The epigenetic regulation of viral genes may be important for the life cycle of EBV. We determined the methylation status of three viral promoters (Wp, Cp, Qp) from EBV B-lymphoblastoid cell lines (LCLs) by pyrosequencing. Our pyrosequencing data showed that the CpG region of Wp was methylated, but the others were not. Interestingly, Wp methylation was increased with proliferation of LCLs. Wp methylation was as high as 74.9% in late-passage LCLs, but 25.6% in early-passage LCLs. From two Burkitt’s lymphoma cell lines, Wp specific hypermethylation was also found (>80%). Interestingly, the expression of EBNA2 gene which located directly next to Wp was associated with its methylation. Our data suggested that Wp specific methylation may be important for the indicator of the proliferation status of LCLs, and the epigenetic viral gene regulation of EBNA2 gene by Wp should be further defined possibly with other biological processes.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.04.169