Serial analysis of gene expression (SAGE) in rat liver regeneration

We have applied serial analysis of gene expression for studying the molecular mechanism of the rat liver regeneration in the model of 70% partial hepatectomy. We generated three SAGE libraries from a normal control liver (NL library: 52,343 tags), from a sham control operated liver (Sham library: 51...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-08, Vol.360 (3), p.545-552
Main Authors: Cimica, Velasco, Batusic, Danko, Haralanova-Ilieva, Borislava, Chen, Yonglong, Hollemann, Thomas, Pieler, Tomas, Ramadori, Giuliano
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Animals
BIOLOGICAL REGENERATION
Computational Biology
CONNECTIVE TISSUE
Connective Tissue Growth Factor
CTGF
Gene Expression Profiling - methods
Gene Expression Regulation, Developmental
GENES
GROWTH FACTORS
HEPATECTOMY
Immediate-Early Proteins - biosynthesis
Immediate-Early Proteins - genetics
Intercellular Signaling Peptides and Proteins - biosynthesis
Intercellular Signaling Peptides and Proteins - genetics
LIVER
Liver - growth & development
Liver - physiology
LIVER CELLS
Liver regeneration
Liver Regeneration - genetics
Male
Partial hepatectomy
PH VALUE
POLYMERASE CHAIN REACTION
RATS
Rats, Inbred F344
Reverse Transcriptase Polymerase Chain Reaction
SAGE
serial analysis of gene expression
Time Factors
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Summary:We have applied serial analysis of gene expression for studying the molecular mechanism of the rat liver regeneration in the model of 70% partial hepatectomy. We generated three SAGE libraries from a normal control liver (NL library: 52,343 tags), from a sham control operated liver (Sham library: 51,028 tags), and from a regenerating liver (PH library: 53,061 tags). By SAGE bioinformatics analysis we identified 40 induced genes and 20 repressed genes during the liver regeneration. We verified temporal expression of such genes by real time PCR during the regeneration process and we characterized 13 induced genes and 3 repressed genes. We found connective tissue growth factor transcript and protein induced very early at 4 h after PH operation before hepatocytes proliferation is triggered. Our study suggests CTGF as a growth factor signaling mediator that could be involved directly in the mechanism of liver regeneration induction.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.06.039