Serial analysis of gene expression (SAGE) in rat liver regeneration

• Published in: Biochemical and biophysical research communications 2007-08, Vol.360 (3), p.545-552
• Main Authors: Cimica, Velasco, Batusic, Danko, Haralanova-Ilieva, Borislava, Chen, Yonglong, Hollemann, Thomas, Pieler, Tomas, Ramadori, Giuliano
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; Animals; BIOLOGICAL REGENERATION; Computational Biology; CONNECTIVE TISSUE; Connective Tissue Growth Factor; CTGF; Gene Expression Profiling - methods; Gene Expression Regulation, Developmental; GENES; GROWTH FACTORS; HEPATECTOMY; Immediate-Early Proteins - biosynthesis; Immediate-Early Proteins - genetics; Intercellular Signaling Peptides and Proteins - biosynthesis; Intercellular Signaling Peptides and Proteins - genetics; LIVER; Liver - growth & development; Liver - physiology; LIVER CELLS; Liver regeneration; Liver Regeneration - genetics; Male; Partial hepatectomy; PH VALUE; POLYMERASE CHAIN REACTION; RATS; Rats, Inbred F344; Reverse Transcriptase Polymerase Chain Reaction; SAGE; serial analysis of gene expression; Time Factors
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2007.06.039

We have applied serial analysis of gene expression for studying the molecular mechanism of the rat liver regeneration in the model of 70% partial hepatectomy. We generated three SAGE libraries from a normal control liver (NL library: 52,343 tags), from a sham control operated liver (Sham library: 51,028 tags), and from a regenerating liver (PH library: 53,061 tags). By SAGE bioinformatics analysis we identified 40 induced genes and 20 repressed genes during the liver regeneration. We verified temporal expression of such genes by real time PCR during the regeneration process and we characterized 13 induced genes and 3 repressed genes. We found connective tissue growth factor transcript and protein induced very early at 4 h after PH operation before hepatocytes proliferation is triggered. Our study suggests CTGF as a growth factor signaling mediator that could be involved directly in the mechanism of liver regeneration induction.