The novel putative bile acid transporter SLC10A5 is highly expressed in liver and kidney

Here we report the identification, cloning, and characterization of SLC10A5, which is a new member of Solute Carrier Family 10 (SLC10), also known as the “sodium/bile acid cotransporter family”. Expression of SLC10A5/Slc10a5 was examined by quantitative real-time PCR and revealed its highest express...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-09, Vol.361 (1), p.26-32
Main Authors: Fernandes, Carla F., Godoy, José R., Döring, Barbara, Cavalcanti, Marcia C.O., Bergmann, Martin, Petzinger, Ernst, Geyer, Joachim
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Amino Acid Sequence
Animals
Bile acid transport
BILE ACIDS
CARRIERS
Cloning, Molecular
ESTRONE
Gene Expression
Humans
HYDROXYANDROSTENONE
IN-SITU HYBRIDIZATION
Kidney - metabolism
LIVER
Liver - metabolism
LIVER CELLS
MICE
Molecular Sequence Data
NTCP
OOCYTES
Oocytes - metabolism
Organic Anion Transporters, Sodium-Dependent - chemistry
Organic Anion Transporters, Sodium-Dependent - genetics
Organic Anion Transporters, Sodium-Dependent - metabolism
POLYMERASE CHAIN REACTION
PROTEINS
Proximal tubules
RATS
SLC10
SLC10A5
SULFATES
Symporters - chemistry
Symporters - genetics
Symporters - metabolism
TUBULES
Xenopus laevis
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Summary:Here we report the identification, cloning, and characterization of SLC10A5, which is a new member of Solute Carrier Family 10 (SLC10), also known as the “sodium/bile acid cotransporter family”. Expression of SLC10A5/Slc10a5 was examined by quantitative real-time PCR and revealed its highest expression levels in liver and kidney in humans, rat and mouse. In rat liver and kidney, Slc10a5 expression was localized by in situ hybridization to hepatocytes and proximal tubules, respectively. A SLC10A5-FLAG fusion protein was expressed in HEK293 cells and showed an apparent molecular weight of 42 kDa after immunoprecipitation. When expressed in Xenopus laevis oocytes, the SLC10A5-FLAG protein was detected in the oocyte’s plasma membrane but showed no transport activity for taurocholate, cholate, estrone-3-sulfate, or dehydroepiandrosterone sulfate. As bile acid carriers are the most related carriers to SLC10A5 though, we strongly suppose that SLC10A5 is an orphan carrier with yet non-identified substrates.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.06.160