NG2 proteoglycan increases mesangial cell proliferation and extracellular matrix production

As a membrane-spanning protein, NG2 chondroitin sulfate proteoglycan interacts with molecules on both sides of plasma membrane. The present study explored the role of NG2 in the pathogenesis of diabetic nephropathy. In the normal kidneys, NG2 was observed predominantly in glomerular mesangium, Bowma...

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Published in:Biochemical and biophysical research communications 2007-10, Vol.361 (4), p.960-967
Main Authors: Xiong, Jing, Wang, Yang, Zhu, Zhonghua, Liu, Jianshe, Wang, Yumei, Zhang, Chun, Hammes, Hans-Peter, Lang, Florian, Feng, Yuxi
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Animals
Antigens - analysis
Antigens - metabolism
Antigens - physiology
Cell Cycle
CELL PROLIFERATION
CHONDROITIN
COLLAGEN
Diabetic Nephropathies - etiology
Diabetic Nephropathies - metabolism
Diabetic Nephropathies - pathology
Diabetic nephropathy
Extracellular matrix
Extracellular Matrix - metabolism
Kidney - chemistry
Kidney - metabolism
Kidney - pathology
KIDNEYS
Male
Mesangial cells
Mesangial Cells - cytology
Mesangial Cells - metabolism
NG2
PATHOGENESIS
Proteoglycans - analysis
Proteoglycans - metabolism
Proteoglycans - physiology
RATS
Rats, Sprague-Dawley
SULFATES
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Summary:As a membrane-spanning protein, NG2 chondroitin sulfate proteoglycan interacts with molecules on both sides of plasma membrane. The present study explored the role of NG2 in the pathogenesis of diabetic nephropathy. In the normal kidneys, NG2 was observed predominantly in glomerular mesangium, Bowman’s capsule and interstitial vessels. Both mRNA and protein expression in kidneys was significantly higher in strepozotocin-induced diabetic rats than that in normal rats. In the cultured rat mesangial cell line HBZY-1, overexpression of NG2 promoted mesangial cell proliferation and extracellular matrix (ECM) production, such as type VI collagen and laminin. Furthermore, target knockdown of NG2 resulted in decreased cell proliferation and ECM formation. The observations suggest that NG2 is up-regulated in diabetic nephropathy. It actively participates in the development and progression of glomerulosclerosis by stimulating proliferation of mesangial cells and deposition of ECM.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.07.113