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Structural and functional studies on Ribonuclease S, retro S and retro-inverso S peptides

Ribonuclease S peptide and S protein offer a unique complementation system to understand the finer features of molecular recognition. In the present study the S peptide (1–16), and its retro and retro-inverso analogs have been analyzed for their structural and biological attributes. RPHPLC, CD, and...

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Published in:	Biochemical and biophysical research communications 2007-12, Vol.364 (3), p.608-613
Main Authors:	Pal-Bhowmick, Ipsita, Pati Pandey, Ramendra, Jarori, Gotam K., Kar, Santosh, Sahal, Dinkar
Format:	Article
Language:	English
Subjects:	60 APPLIED LIFE SCIENCES Amino Acid Sequence ANTIBODIES ANTIGENS Binding Sites CELL PROLIFERATION CYTIDINE DICHROISM ENZYME IMMUNOASSAY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY Mimetics NITRIC OXIDE NMR NUCLEAR MAGNETIC RESONANCE OVERHAUSER EFFECT Peptide Fragments - chemistry Peptide Fragments - immunology Peptide Fragments - ultrastructure PEPTIDES Protein Binding Retro/retro-inverso peptides Ribonuclease Ribonucleases - chemistry Ribonucleases - immunology Ribonucleases - ultrastructure RNA-ASE S peptide Structure-Activity Relationship T cells T-Lymphocytes - immunology
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description	Ribonuclease S peptide and S protein offer a unique complementation system to understand the finer features of molecular recognition. In the present study the S peptide (1–16), and its retro and retro-inverso analogs have been analyzed for their structural and biological attributes. RPHPLC, CD, and NMR analyses have revealed that the physicochemical and conformational properties of the S peptide are distinct from those of its retro and retro-inverso analogs. On the functional side, while the S peptide complemented the S protein to give RNase activity, was recognized by anti-S peptide antibodies and induced T cell proliferation, neither the retro nor the retro-inverso S peptides could do so.
doi_str_mv	10.1016/j.bbrc.2007.10.056
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subjects	60 APPLIED LIFE SCIENCES Amino Acid Sequence ANTIBODIES ANTIGENS Binding Sites CELL PROLIFERATION CYTIDINE DICHROISM ENZYME IMMUNOASSAY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY Mimetics NITRIC OXIDE NMR NUCLEAR MAGNETIC RESONANCE OVERHAUSER EFFECT Peptide Fragments - chemistry Peptide Fragments - immunology Peptide Fragments - ultrastructure PEPTIDES Protein Binding Retro/retro-inverso peptides Ribonuclease Ribonucleases - chemistry Ribonucleases - immunology Ribonucleases - ultrastructure RNA-ASE S peptide Structure-Activity Relationship T cells T-Lymphocytes - immunology
title	Structural and functional studies on Ribonuclease S, retro S and retro-inverso S peptides
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