Correlation between proliferative activity and cellular thickness of human mesenchymal stem cells

A cell’s shape is known to be related to its proliferative activity. In particular, large and flat mammalian adult stem cells seem to show slow proliferation, however using quantitative analysis to prove the phenomenon is difficult. We measured the proliferation and cellular thickness of human mesen...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-04, Vol.368 (2), p.256-260
Main Authors: Katsube, Yoshihiro, Hirose, Motohiro, Nakamura, Chikashi, Ohgushi, Hajime
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ATOMIC FORCE MICROSCOPY
CELL PROLIFERATION
Cell Size
Cells, Cultured
Cellular senescence
Cellular thickness
DRUGS
GALACTOSIDASE
GENES
Humans
MEDICINE
Mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Proliferative activity
Regenerative medicine
Statistics as Topic
STEM CELLS
THICKNESS
Tissue Engineering - methods
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Summary:A cell’s shape is known to be related to its proliferative activity. In particular, large and flat mammalian adult stem cells seem to show slow proliferation, however using quantitative analysis to prove the phenomenon is difficult. We measured the proliferation and cellular thickness of human mesenchymal stem cells (MSCs) by atomic force microscopy and found that MSCs with high proliferative activity were thick while those with low proliferative activity were thin, even though these MSCs were early passage cells. Further, low proliferative MSCs contained many senescence-associated β-galactosidase positive cells together with high senescence-associated gene expression. These findings suggest that the measurement of cellular thickness is useful for estimating the proliferative activity of human MSCs and is expected to be a practical tool for MSC applications in regenerative medicine.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.01.051