Autographa californica multiple nucleopolyhedrovirus ac142 , a core gene that is essential for BV production and ODV envelopment

Abstract Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac142 is a baculovirus core gene and encodes a protein previously shown to associate with occlusion-derived virus (ODV). To determine its role in the baculovirus life cycle, we used the AcMNPV bacmid system to generate an ac142 d...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2008-03, Vol.372 (2), p.325-339
Main Authors: McCarthy, Christina B, Dai, Xiaojiang, Donly, Cam, Theilmann, David A
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ac142
Animals
Autographa californica multiple nucleopolyhedrovirus
Cell Line
Core gene
CYTOPLASM
DNA REPLICATION
ELECTRON MICROSCOPY
Envelopment
FLUORESCENCE
Gene Deletion
Gene Expression Regulation, Viral
GENES
Genes, Essential - genetics
Genes, Viral - genetics
Infectious Disease
LIFE CYCLE
Nucleocapsid
Nucleocapsid - ultrastructure
Nucleopolyhedrovirus - genetics
Nucleopolyhedrovirus - growth & development
Nucleopolyhedrovirus - ultrastructure
Occlusion body
ODV
PROTEINS
Spodoptera - cytology
TITRATION
transcription (genetics)
Transcription, Genetic
viral proteins
Viral Proteins - genetics
Viral Proteins - metabolism
Virus Diseases
virus replication
Virus Replication - genetics
VIRUSES
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Summary:Abstract Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac142 is a baculovirus core gene and encodes a protein previously shown to associate with occlusion-derived virus (ODV). To determine its role in the baculovirus life cycle, we used the AcMNPV bacmid system to generate an ac142 deletion virus (AcBAC ac142 KO-PH-GFP ). Fluorescence and light microscopy revealed that AcBAC ac142 KO-PH-GFP exhibits a single-cell infection phenotype. Titration assays and Western blot confirmed that AcBAC ac142 KO-PH-GFP is unable to produce budded virus (BV). However, viral DNA replication is unaffected and the development of occlusion bodies in AcBAC ac142 KO-PH-GFP -transfected cells evidenced progression to very late phases of the viral infection. Western blot analysis showed that AC142 is expressed in the cytoplasm and nucleus throughout infection and that it is a structural component of BV and ODV which localizes to nucleocapsids. Electron microscopy indicates that ac142 is required for nucleocapsid envelopment to form ODV and their subsequent occlusion, a fundamental process to all baculoviruses.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2007.10.019