Tyk2 expression and its signaling enhances the invasiveness of prostate cancer cells

Protein tyrosine kinase plays a central role in the proliferation and differentiation of various types of cells. One of these protein kinases, Tyk2, a member of the Jak family kinases, is known to play important roles in receptor signal transduction by interferons, interleukins, growth factors, and...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-05, Vol.369 (2), p.292-296
Main Authors: Ide, Hisamitsu, Nakagawa, Takashi, Terado, Yuichi, Kamiyama, Yutaka, Muto, Satoru, Horie, Shigeo
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Cell Line
CELL PROLIFERATION
Gene Expression Regulation, Neoplastic
Humans
INTERFERON
Invasion
Jak
Male
METASTASES
Metastasis
Neoplasm Invasiveness
NEOPLASMS
PHOSPHOTRANSFERASES
PLASMINOGEN
POLYMERASE CHAIN REACTION
PROSTATE
Prostate cancer
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
RECEPTORS
RNA
Signal Transduction
TRANSCRIPTION
Tyk2
TYK2 Kinase - metabolism
TYROSINE
UROKINASE
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Summary:Protein tyrosine kinase plays a central role in the proliferation and differentiation of various types of cells. One of these protein kinases, Tyk2, a member of the Jak family kinases, is known to play important roles in receptor signal transduction by interferons, interleukins, growth factors, and other hormones. In the present study, we investigated Tyk2 expression and its role in the growth and invasiveness of human prostate cancer cells. We used a small interfering RNA targeting Tyk2 and an inhibitor of Tyk2, tyrphostin A1, to suppress the expression and signaling of Tyk2 in prostate cancer cells. We detected mRNAs for Jak family kinases in prostate cancer cell lines by RT-PCR and Tyk2 protein in human prostate cancer specimens by immunohistochemistry. Inhibition of Tyk2 signaling resulted in attenuation of the urokinase-type plasminogen activator-enhanced invasiveness of prostate cancer cells in vitro without affecting the cellular growth rate. These results suggest that Tyk2 signaling in prostate cancer cells facilitate invasion of these cells, and interference with this signaling may be a potential therapeutic pathway.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.08.160