HoxD10 gene delivery using adenovirus/adeno-associate hybrid virus inhibits the proliferation and tumorigenicity of GH4 pituitary lactotrope tumor cells

Prolactinoma is one of the most common types of pituitary adenoma. It has been reported that a variety of growth factors and cytokines regulating cell growth and angiogenesis play an important role in the growth of prolactinoma. HoxD10 has been shown to impair endothelial cell migration, block angio...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-07, Vol.371 (3), p.371-374
Main Authors: Cho, Mi Ae, Yashar, Parham, Kim, Suk Kyoung, Noh, Taewoong, Gillam, Mary P., Lee, Eun Jig, Jameson, J. Larry
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Adeno-associated virus
ADENOMAS
Adenoviridae - genetics
ADENOVIRUS
Adenovirus/adeno-associate virus hybrid vector
Angiogenesis
Animals
beta-Galactosidase - genetics
CELL PROLIFERATION
Cyclin D2
Cyclins - metabolism
Dependovirus - genetics
Fibroblast growth factor 2
Fibroblast Growth Factor 2 - metabolism
FIBROBLASTS
GALACTOSIDASE
Gene Transfer Techniques
GENES
Genetic Therapy
Genetic Vectors
GH4 lactotrope tumor
Homeodomain Proteins - genetics
HoxD10
IN VITRO
LYMPHOKINES
MICE
Pituitary Neoplasms - therapy
Prolactinoma - therapy
RATS
Transcription Factors - genetics
TUMOR CELLS
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Summary:Prolactinoma is one of the most common types of pituitary adenoma. It has been reported that a variety of growth factors and cytokines regulating cell growth and angiogenesis play an important role in the growth of prolactinoma. HoxD10 has been shown to impair endothelial cell migration, block angiogenesis, and maintain a differentiated phenotype of cells. We investigated whether HoxD10 gene delivery could inhibit the growth of prolactinoma. Rat GH4 lactotrope tumor cells were infected with adenovirus/adeno-associated virus (Ad/AAV) hybrid vectors carrying the mouse HoxD10 gene (Hyb-HoxD10) or the β-galactosidase gene (Hyb-Gal). Hyb-HoxD10 expression inhibited GH4 cell proliferation in vitro. The expression of FGF-2 and cyclin D2 was inhibited in GH4 cells infected with Hyb-HoxD10. GH4 cells transduced with Hyb-HoxD10 did not form tumors in nude mice. These results indicate that the delivery of HoxD10 could potentially inhibit the growth of PRL-secreting tumors. This approach may be a useful tool for targeted therapy of prolactinoma and other neoplasms.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.04.085