Novel agmatine analogue, {gamma}-guanidinooxypropylamine (GAPA) efficiently inhibits proliferation of Leishmania donovani by depletion of intracellular polyamine levels

• Published in: Biochemical and biophysical research communications 2008-10, Vol.375 (1)
• Main Authors: Singh, Sushma, Jhingran, Anupam, Sharma, Ankur, Simonian, Alina R., Soininen, Pasi, Vepsalainen, Jouko, Khomutov, Alex R., Madhubala, Rentala
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; DECARBOXYLASES; LEAD COMPOUNDS; ORNITHINE; PARASITES; PH VALUE; PUTRESCINE; SPERMIDINE
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2008.07.143

The efficacy of {gamma}-guanidinooxypropylamine (GAPA), a novel agmatine analogue against protozoan parasite, Leishmaniadonovani was evaluated. Wild-type and ornithine decarboxylase-overexpressors of L. donovani were used to study the effect and mode of action of this inhibitor. GAPA inhibited the growth of both promastigotes and amastigotes. Ornithine decarboxylase (ODC) activity and polyamine levels were markedly lower in cells treated with GAPA and proliferation was rescued by addition of putrescine or spermidine. GAPA inhibited L. donovani recombinant ODC with K{sub i} value of {approx}60 {mu}M. The ODC-overexpressors showed significant resistance to GAPA. GAPA has pK{sub a} 6.71 and at physiological pH the analogue can mimic protonated state of putrescine and can probably use putrescine transport system. Transport of putrescine in wild-type L. donovani promastigotes was inhibited by GAPA. We for the first time report that GAPA is a potential antileishmanial lead compound and it possibly inhibits L. donovani growth by depletion of intracellular polyamine levels.