Expression profile analysis of aorta-gonad-mesonephros region-derived stromal cells reveals genes that regulate hematopoiesis

The aorta-gonad-mesonephros (AGM) region is involved in the generation and maintenance of the first definitive hematopoietic stem cells (HSCs). A mouse AGM-derived cell line, AGM-S3, was shown to support the development of HSCs. To elucidate the molecular mechanisms regulating early hematopoiesis, w...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-12, Vol.377 (1), p.205-209
Main Authors: Nagao, Kenji, Ohta, Takayuki, Hinohara, Atsushi, Tahara, Tomoyuki, Hagiwara, Tetsuya, Maeda, Yoshitake, Yoneya, Takashi, Sohma, Yoshiaki, Heike, Toshio, Nakahata, Tatsutoshi, Inagaki, Yoshimasa, Nishikawa, Mitsuo
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
AGM
Animals
AORTA
Aorta - cytology
Aorta - embryology
Aorta - metabolism
BLOOD
BLOOD FORMATION
Cell Line
CELL PROLIFERATION
Fetal Blood - cytology
Fetal Blood - metabolism
Gene Expression Profiling
Gene Expression Regulation, Developmental
GENES
Glypican
Glypicans - genetics
Glypicans - physiology
GONADS
Gonads - cytology
Gonads - embryology
Gonads - metabolism
Hematopoiesis - genetics
Hematopoietic stem cell
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - metabolism
HEMATOPOIETIC SYSTEM
Humans
Mesonephros - cytology
Mesonephros - metabolism
MICE
STEM CELLS
Stroma
Stromal Cells - cytology
Stromal Cells - metabolism
Transfection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aorta-gonad-mesonephros (AGM) region is involved in the generation and maintenance of the first definitive hematopoietic stem cells (HSCs). A mouse AGM-derived cell line, AGM-S3, was shown to support the development of HSCs. To elucidate the molecular mechanisms regulating early hematopoiesis, we obtained subclones from AGM-S3, one of which was hematopoiesis supportive (S3-A9) and the other one of which was non-supportive (S3-A7), and we analyzed their gene expression profiles by gene chip analysis. In the present study, we found that Glypican-1 (GPC1) was highly expressed in the supportive subclone AGM-S3-A9. Over-expression of GPC1 in non-supportive cells led to the proliferation of progenitor cells in human cord blood when cocultured with the transfected-stromal cells. Thus, GPC1 may have an important role in the establishment of a microenvironment that supports early events in hematopoiesis.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.09.123