Impact of cranberry on Escherichia coli cellular surface characteristics

The anti-adhesive effects of cranberry have been attributed to both interactions of its components with the surface of bacterial cells and to inhibition of p-fimbriae expression. Previous reports also suggested that the presence of cranberry juice changed the Gram stain characteristics of Escherichi...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-12, Vol.377 (3), p.992-994
Main Authors: Johnson, Brandy J., Lin, Baochuan, Dinderman, Michael A., Rubin, Robert A., Malanoski, Anthony P., Ligler, Frances S.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ADHESIVES
Anti-adhesive
Antibacterial
Bacterial Adhesion - drug effects
Bacterial Adhesion - physiology
BERRIES
Cell Membrane - drug effects
Cell Membrane - physiology
Cell Membrane - ultrastructure
Cranberry
ESCHERICHIA COLI
Escherichia coli - drug effects
Escherichia coli - physiology
Escherichia coli - ultrastructure
GENES
INHIBITION
IODINE
MEMBRANES
Microscopy, Electron, Scanning
MORPHOLOGY
Plant Extracts - chemistry
Plant Extracts - pharmacology
Proanthocyandin
PROTEINS
Receptors, Immunologic - drug effects
Receptors, Immunologic - physiology
Receptors, Immunologic - ultrastructure
SCANNING ELECTRON MICROSCOPY
STAINS
SURFACES
Vaccinium macrocarpon
Vaccinium macrocarpon - chemistry
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Summary:The anti-adhesive effects of cranberry have been attributed to both interactions of its components with the surface of bacterial cells and to inhibition of p-fimbriae expression. Previous reports also suggested that the presence of cranberry juice changed the Gram stain characteristics of Escherichia coli. Here, we show that the morphology of E. coli is changed when grown in the presence of juice or extract from Vaccinium macrocarpon (cranberry). Gene expression analysis indicates the down regulation of flagellar basal body rod and motor proteins. Consistent with this finding and previous reports, the SEM images indicate a decrease in the visible p-fimbriae. The iodine used in Gram-staining protocols was found to interact differently with the bacterial membrane when cells were cultured in spiked media. Slight alterations in the Gram stain protocol demonstrated that culturing in the presence of cranberry juice does not change the Gram stain characteristics contradicting other reports.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.10.098