Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana , cucurbits, and legumes

• Published in: Virology (New York, N.Y.) N.Y.), 2009-04, Vol.386 (2), p.407-416
• Main Authors: Igarashi, Aki, Yamagata, Kousuke, Sugai, Tomokazu, Takahashi, Yukari, Sugawara, Emiko, Tamura, Akihiro, Yaegashi, Hajime, Yamagishi, Noriko, Takahashi, Tsubasa, Isogai, Masamichi, Takahashi, Hideki, Yoshikawa, Nobuyuki
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; ANTIGENS; Apple latent spherical virus; Apple latent spherical virus vector; ARABIDOPSIS; Arabidopsis - genetics; Arabidopsis thaliana; Cheravirus; Citrullus lanatus; Cucumis melo; Cucumis sativus; Cucurbit; Cucurbita pepo; Cucurbitaceae - genetics; Fabaceae - genetics; FOOD; FRUITS; Gene Expression Regulation, Plant; Gene Knockout Techniques; Gene Silencing; GENES; Genetic Engineering - methods; Genetic Vectors; Glycine max; GROWTH; host-pathogen relationships; Infectious Disease; Lagenaria siceraria; Legume; legumes; Luffa cylindrical; Lyases - genetics; Lycopersicon esculentum; MAGNOLIOPHYTA; MAGNOLIOPSIDA; Malus; MERISTEMS; MICROORGANISMS; Nicotiana - genetics; Nicotiana benthamiana; Nicotiana glutinosa; Nicotiana occidentalis; Nicotiana tabacum; Oxidoreductases - genetics; PARASITES; PHENOTYPE; Pisum sativum; PLANT GROWTH; PLANT TISSUES; plant viruses; PLANTS; positive sense, single-stranded RNA viruses; RNA Viruses - genetics; RNA, Plant - genetics; Secoviridae; Solanum lycopersicum - genetics; TOBACCO; TOMATOES; Vigna angularis; Vigna unguiculata; Virus-induced gene silencing; VIRUSES
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2009.01.039

Abstract Apple latent spherical virus (ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a subunit of magnesium chelatase ( SU ) and phytoene desaturase ( PDS ) genes induced highly uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis thaliana , and economically important crops such as tomato, legume, and cucurbit species. The silencing phenotypes persisted throughout plant growth in these plants. In addition, ALSV vectors could be successfully used to silence a meristem gene, proliferating cell nuclear antigen and disease resistant N gene in tobacco and RCY1 gene in A. thaliana . As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species.