HIF-1{alpha} is necessary to support gluconeogenesis during liver regeneration

Coordinated recovery of hepatic glucose metabolism is prerequisite for normal liver regeneration. To examine roles of hypoxia inducible factor-1{alpha} (HIF-1{alpha}) for hepatic glucose homeostasis during the reparative process, we inactivated the gene in hepatocytes in vivo. Following partial hepa...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2009-10, Vol.387 (4)
Main Authors: Tajima, Toshihide, Goda, Nobuhito, Fujiki, Natsuko, Hishiki, Takako, Nishiyama, Yasumasa, Senoo-Matsuda, Nanami, Shimazu, Motohide, Soga, Tomoyoshi, Yoshimura, Yasunori, Johnson, Randall S., Suematsu, Makoto
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ALANINES
BLOOD
FRUCTOSE
GLUCOSE
GLYCOGEN
HEPATECTOMY
HOMEOSTASIS
LIVER
LIVER CELLS
MICE
MUTANTS
PHOSPHATES
PHOSPHOENOLPYRUVATE
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Summary:Coordinated recovery of hepatic glucose metabolism is prerequisite for normal liver regeneration. To examine roles of hypoxia inducible factor-1{alpha} (HIF-1{alpha}) for hepatic glucose homeostasis during the reparative process, we inactivated the gene in hepatocytes in vivo. Following partial hepatectomy (PH), recovery of residual liver weight was initially retarded in the mutant mice by down-regulation of hepatocyte proliferation, but occurred comparably between the mutant and control mice at 72 h after PH. At this time point, the mutant mice showed lowered blood glucose levels with enhanced accumulation of glycogen in the liver. The mutant mice exhibited impairment of hepatic gluconeogenesis as assessed by alanine tolerance test. This appeared to result from reduced expression of PGK-1 and PEPCK since 3-PG, PEP and malate were accumulated to greater extents in the regenerated liver. In conclusion, these findings provide evidence for roles of HIF-1{alpha} in the regulation of gluconeogenesis under liver regeneration.
ISSN:0006-291X
1090-2104
DOI:10.1016/J.BBRC.2009.07.115